ArticlePlant methods2020
Simple semi-high throughput determination of activity signatures of key antioxidant enzymes for physiological phenotyping.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 90 citations in OpenAlex.
- The Dark Side of Antioxidants: When Scavenging ROS Undermines Plant Stress Acclimation.Antioxidants (Basel, Switzerland) · 2026Review
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- Tolerance and Antioxidant Activity of Watermelon Cultivars Pre-Treated with Stress Attenuators and Subjected to Water Deficit.Plants (Basel, Switzerland) · 2026Article
- Novel halotolerant fungal endophytes from hypersaline coastal ecosystems as sustainable biostimulants for early growth performance of tomato and wheat under saline stress.Frontiers in plant science · 2026Article
- Proline-Nitrogen Metabolic Coordination Mediates Cold Priming-Induced Freezing Tolerance in Maize.Plants (Basel, Switzerland) · 2025Article
- The effect of nano‑selenium application on the quality and flavor of kiwifruit.Food chemistry: X · 2025Article
- Antioxidant Responses and Redox Regulation Within Plant-Beneficial Microbe Interaction.Antioxidants (Basel, Switzerland) · 2024Review
- The Optimum Mixed Cropping Ratio of Oat and Alfalfa Enhanced Plant Growth, Forage Yield, and Forage Quality in Saline Soil.Plants (Basel, Switzerland) · 2024Article
- Seed Treatment with Cold Plasma and Electromagnetic Field: Changes in Antioxidant Capacity of Seedlings in DifferentPlants (Basel, Switzerland) · 2024Article
- An Innovative Approach to Alleviate Zinc Oxide Nanoparticle Stress on Wheat through Nanobubble Irrigation.International journal of molecular sciences · 2024Article
- Preharvest 24-epibrassinolide treatment prolongs harvest duration and shelf life in sweet corn.Food chemistry. Molecular sciences · 2023Article
- Unveiling plant defense arsenal: metabolic strategies inHorticulture research · 2023Article
- Abscisic Acid Regulates Carbohydrate Metabolism, Redox Homeostasis and Hormonal Regulation to Enhance Cold Tolerance in Spring Barley.International journal of molecular sciences · 2023Article
- Phytomediated Silver Nanoparticles (AgNPs) Embellish Antioxidant Defense System, Ameliorating HLB-Diseased 'Kinnow' Mandarin Plants.Molecules (Basel, Switzerland) · 2023Article
- Candidate pathogenicity factor/effector proteins of 'Frontiers in plant science · 2023Article
- Silicon-mediated herbivore defence in a pasture grass under reduced and Anthropocene levels of COFrontiers in plant science · 2023Article
- Functional phenomics for improved climate resilience in Nordic agriculture.Journal of experimental botany · 2022Review
- High-throughput phenotyping of physiological traits for wheat resilience to high temperature and drought stress.Journal of experimental botany · 2022Article
- Exogenous Melatonin Reprograms the Rhizosphere Microbial Community to Modulate the Responses of Barley to Drought Stress.International journal of molecular sciences · 2022Article
- Phytogenic Selenium Nanoparticles Elicited the Physiological, Biochemical, and Antioxidant Defense System Amelioration of Huanglongbing-Infected 'Kinnow' Mandarin Plants.Nanomaterials (Basel, Switzerland) · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundReactive oxygen species (ROS) such as hydrogen peroxide and superoxide anions significantly accumulate during biotic and abiotic stress and cause oxidative damage and eventually cell death. There is accumulating evidence that ROS are also involved in regulating beneficial plant-microbe interactions, signal transduction and plant growth and development. Due to the relevance of ROS throughout the life cycle and for interaction with the multifactorial environment, the physiological phenotyping of the mechanisms controlling ROS homeostasis is of general importance.
resultsIn this study, we have developed a robust and resource-efficient experimental platform that allows the determination of the activities of the nine key ROS scavenging enzymes from a single extraction that integrates posttranscriptional and posttranslational regulations. The assays were optimized and adapted for a semi-high throughput 96-well assay format. In a case study, we have analyzed tobacco leaves challenged by pathogen infection, drought and salt stress. The three stress factors resulted in distinct activity signatures with differential temporal dynamics.
conclusionsThis experimental platform proved to be suitable to determine the antioxidant enzyme activity signature in different tissues of monocotyledonous and dicotyledonous model and crop plants. The universal enzymatic extraction procedure combined with the 96-well assay format demonstrated to be a simple, fast and semi-high throughput experimental platform for the precise and robust fingerprinting of nine key antioxidant enzymatic activities in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.