ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2020
Identification and validation of superior housekeeping gene(s) for qRT-PCR data normalization in
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Identification of housekeeping gene for future studies exploring effects of cryopreservation on gene expression in shrimp.Scientific reports · 2025Article
- Identifying the stability of housekeeping genes to be used for the quantitative real-time PCR normalization in retinal tissue of streptozotocin-induced diabetic rats.International journal of ophthalmology · 2024Article
- Transcriptome Mining Provides Insights into Cell Wall Metabolism and Fiber Lignification inPlants (Basel, Switzerland) · 2022Article
- Selection and stability validation of reference gene candidates for transcriptional analysis in Rousettus aegyptiacus.Scientific reports · 2021Article
- Combining transcriptome analysis and GWAS for identification and validation of marker genes in thePeerJ · 2021Article
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Authors and funding
8 authors.
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Abstract
The adaptive mechanisms in Agave species enable them to survive and exhibit remarkable tolerance to abiotic stresses. Quantitative real-time PCR is a highly reliable approach for validation of targeted differential gene expression. However, stable housekeeping gene(s) is prerequisite for accurate normalization of expression data by qRT-PCR. Till date, no systematic validation study for candidate housekeeping gene identification or evaluation has been carried-out in Agave species. A total of 17 candidate housekeeping genes were identified from the de novo assembled transcriptomic data of
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