ArticleNature chemical biology2020
An aminoacylation ribozyme evolved from a natural tRNA-sensing T-box riboswitch.
Article in Nature chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.
- Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides.Frontiers in bioengineering and biotechnology · 2020Pooled it
- Artificial initiation codons and engineered initiator tRNAs enable N-terminal noncanonical amino acid incorporation in intact cell-free translation systems.bioRxiv : the preprint server for biology · 2026Article
- Article
- Reprogramming the genetic code with flexizymes.Nature reviews. Chemistry · 2024Review
- A potential role for RNA aminoacylation prior to its role in peptide synthesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Primordial aminoacyl-tRNA synthetases preferred minihelices to full-length tRNA.Nucleic acids research · 2024Article
- Engineering tRNAs for the Ribosomal Translation of Non-proteinogenic Monomers.Chemical reviews · 2024Review
- An anticodon-sensing T-boxzyme generates the elongator nonproteinogenic aminoacyl-tRNA in situ of a custom-made translation system for incorporation.Nucleic acids research · 2024Article
- On the origin of life: an RNA-focused synthesis and narrative.RNA (New York, N.Y.) · 2023Article
- Catalytic mechanism and pH dependence of a methyltransferase ribozyme (MTR1) from computational enzymology.Nucleic acids research · 2023Article
- Structure-based investigations of the NAD+-II riboswitch.Nucleic acids research · 2023Article
- Review
- Structure and mechanism of the methyltransferase ribozyme MTR1.Nature chemical biology · 2022Article
- Development and characterization of a glycine biosensor system for fine-tuned metabolic regulation in Escherichia coli.Microbial cell factories · 2022Article
- Nonenzymatic assembly of active chimeric ribozymes from aminoacylated RNA oligonucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A natural riboswitch scaffold with self-methylation activity.Nature communications · 2021Article
- TBDB: a database of structurally annotated T-box riboswitch:tRNA pairs.Nucleic acids research · 2021Article
- Unboxing the T-box riboswitches-A glimpse into multivalent and multimodal RNA-RNA interactions.Wiley interdisciplinary reviews. RNA · 2020Review
- Rational Design of Aptamer-Tagged tRNAs.International journal of molecular sciences · 2020Article
- Imaginary Ribozymes.ACS chemical biology · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When the primitive translation system first emerged in the hypothetical RNA world, ribozymes could have been responsible for aminoacylation. Given that naturally occurring T-box riboswitches selectively sense the aminoacylation status of cognate tRNAs, we introduced a domain of random sequence into a T-box-tRNA conjugate and isolated ribozymes that were self-aminoacylating on the 3'-terminal hydroxyl group. One of them, named Tx2.1, recognizes the anticodon and D-loop of tRNA via interaction with its stem I domain, similarly to the parental T-box, and selectively charges N-biotinyl-L-phenylalanine (Bio-
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