ArticleNature methods2020
RecV recombinase system for in vivo targeted optogenomic modifications of single cells or cell populations.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 50 citations in OpenAlex.
- Materialogenetics: an emerging and promising framework for cell-specific genetic manipulation.Bioactive materials · 2026Article
- Enhancing the performance of Magnets photosensors.Nature communications · 2026Article
- A chemically inducible, leakless Cre recombinase by split-protein-based efficient and enhanced degradation (SPEED).Communications biology · 2025Article
- Efficient site-specific recombination by self-activating split-Dre recombinase in mammalian cells and E. coli.Journal of biological engineering · 2025Article
- A rapid and efficient red-light-activated Cre recombinase system for genome engineering in mammalian cells and transgenic mice.Nucleic acids research · 2025Article
- Activating UCHL1 through the CRISPR activation system promotes cartilage differentiation mediated by HIF-1α/SOX9.Journal of cellular and molecular medicine · 2024Article
- A noninvasive photoactivatable split-Cre recombinase system for genome engineering in zebrafish.iScience · 2024Article
- Reprogramming a Doxycycline-Inducible Gene Switch System for Bacteria-Mediated Cancer Therapy.Molecular imaging and biology · 2024Article
- A Novel Flp Reporter Mouse Shows That TRPA1 Expression Is Largely Limited to Sensory Neuron Subsets.eNeuro · 2023Article
- A toolbox of astrocyte-specific, serotype-independent adeno-associated viral vectors using microRNA targeting sequences.Nature communications · 2023Article
- Optical opening of the blood-brain barrier for targeted and ultra-sparse viral infection of cells in mouse cortex.Cell reports methods · 2023Article
- Review
- Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cells.Cell structure and function · 2023Article
- Adult tissue-specific stem cell interaction: novel technologies and research advances.Frontiers in cell and developmental biology · 2023Review
- LOV2-based photoactivatable CaMKII and its application to single synapses: Local Optogenetics.Biophysics and physicobiology · 2023Article
- Precise modulation of embryonic development through optogenetics.Genesis (New York, N.Y. : 2000) · 2022Review
- Stable Transgenic Mouse Strain with Enhanced Photoactivatable Cre Recombinase for Spatiotemporal Genome Manipulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Optogenetics for transcriptional programming and genetic engineering.Trends in genetics : TIG · 2022Review
- A red light-responsive photoswitch for deep tissue optogenetics.Nature biotechnology · 2022Article
- Article
Corrections and comments
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Authors and funding
22 authors at 6 institutions in 2 countries.
Funding
Abstract
Brain circuits comprise vast numbers of interconnected neurons with diverse molecular, anatomical and physiological properties. To allow targeting of individual neurons for structural and functional studies, we created light-inducible site-specific DNA recombinases based on Cre, Dre and Flp (RecVs). RecVs can induce genomic modifications by one-photon or two-photon light induction in vivo. They can produce targeted, sparse and strong labeling of individual neurons by modifying multiple loci within mouse and zebrafish genomes. In combination with other genetic strategies, they allow intersectional targeting of different neuronal classes. In the mouse cortex they enable sparse labeling and whole-brain morphological reconstructions of individual neurons. Furthermore, these enzymes allow single-cell two-photon targeted genetic modifications and can be used in combination with functional optical indicators with minimal interference. In summary, RecVs enable spatiotemporally precise optogenomic modifications that can facilitate detailed single-cell analysis of neural circuits by linking genetic identity, morphology, connectivity and function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.