ArticleNature methods2020
Single-molecule displacement mapping unveils nanoscale heterogeneities in intracellular diffusivity.
Article in Nature methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 139 citations in OpenAlex.
- Mapping Nanoscale Forces and Potentials in Live Cells with Microsecond 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Cancer Heterogeneity and Cancer Cell Plasticity: Molecular Mechanisms and Precision Therapy.MedComm · 2026Review
- ErbB family receptor dimerization dynamics and dysregulation via long-term single-molecule imaging.Cell · 2026Article
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- 3D positioning and optically detected magnetic resonance of intracellular fluorescent nanodiamonds using a multi-plane microscope.Biophysics reports · 2026Article
- Structural and functional implications of phase separation of membrane protein LacY in Escherichia coli.Nature communications · 2026Article
- Volumetric Single-Molecule Tracking Inside Subcellular Structures.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
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- D‑Blur: A Deep Learning Approach for Mapping Subdiffraction Diffusion with Motion-Blurred Images.Chemical & biomedical imaging · 2025Article
- Single-molecule live-cell RNA imaging with CRISPR-Csm.Nature biotechnology · 2025Article
- Direct microsecond wide-field single-molecule tracking and super-resolution mapping via CCD vertical shift.Nature communications · 2025Article
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- Super-resolution algorithms for imaging FCS enhancement: A comparative study.Biophysical journal · 2025Article
- Fast and robust drift correction for single-molecule localization microscopy.Nature communications · 2025Article
- Quantifying the Shape and Stiffness of Single Extracellular Vesicles in Aqueous Solution via Membrane Diffusivity Measurements.Chemical & biomedical imaging · 2025Article
- Short activation domains control chromatin association of transcription factors.bioRxiv : the preprint server for biology · 2025Article
- Local mapping of the nanoscale viscoelastic properties of fluid membranes by AFM nanorheology.Nature communications · 2025Article
- Super-Resolution Mapping and Quantification of Molecular Diffusion via Single-Molecule Displacement/Diffusivity Mapping (SMAccounts of chemical research · 2025Article
- DNA Origami Multicolor Quantum Dot Platforms for Sub-diffraction Spectral Separation Imaging.Methods in molecular biology (Clifton, N.J.) · 2025Article
12 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Intracellular diffusion underlies vital cellular processes. However, it remains difficult to elucidate how an unbound protein diffuses inside the cell with good spatial resolution and sensitivity. Here we introduce single-molecule displacement/diffusivity mapping (SMdM), a super-resolution strategy that enables the nanoscale mapping of intracellular diffusivity through local statistics of the instantaneous displacements of freely diffusing single molecules. We thus show that the diffusion of an average-sized protein in the mammalian cytoplasm and nucleus is spatially heterogeneous at the nanoscale, and that variations in local diffusivity correlate with the ultrastructure of the actin cytoskeleton and the organization of the genome, respectively. SMdM of differently charged proteins further unveils that the possession of positive, but not negative, net charges drastically impedes diffusion, and that the rate is determined by the specific subcellular environments. We thus unveil rich heterogeneities and charge effects in intracellular diffusion at the nanoscale.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.