ArticleJournal of experimental botany2020
Arabidopsis ECHIDNA protein is involved in seed coloration, protein trafficking to vacuoles, and vacuolar biogenesis.
Article in Journal of experimental botany, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Arabidopsis ECHIDNA Plays an Important Role in Auxin Regulation of Clathrin-Mediated Trafficking During Root Gravitropism.Plant, cell & environment · 2026Article
- Post-Golgi Trafficking in Plant Cells.Sub-cellular biochemistry · 2026Review
- Overexpression of OsDUF846.2 enhances the sensitivity of rice to salt and heat stresses.BMC plant biology · 2025Article
- A Genome-Wide Association Screen for Genes Affecting Leaf Trichome Development and Epidermal Metal Accumulation in Arabidopsis.Plant, cell & environment · 2025Article
- Proanthocyanidin biosynthesis and postharvest seed coat darkening in pinto bean.Phytochemistry reviews : proceedings of the Phytochemical Society of Europe · 2025Review
- Single-cell transcriptomic analysis reveals the developmental trajectory and transcriptional regulatory networks of quinoa salt bladders.Stress biology · 2024Article
- The complexities of proanthocyanidin biosynthesis and its regulation in plants.Plant communications · 2023Review
- The plant trans-Golgi network component ECHIDNA regulates defense, cell death, and endoplasmic reticulum stress.Plant physiology · 2023Article
- Biochemistry and Molecular Basis of Intracellular Flavonoid Transport in Plants.Plants (Basel, Switzerland) · 2022Review
- EPSIN1 and MTV1 define functionally overlapping but molecularly distinctProceedings of the National Academy of Sciences of the United States of America · 2020Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavonoids are a major group of plant-specific metabolites that determine flower and seed coloration. In plant cells, flavonoids are synthesized at the cytosolic surface of the endoplasmic reticulum and are sequestered in the vacuole. It is possible that membrane trafficking, including vesicle trafficking and organelle dynamics, contributes to flavonoid transport and accumulation. However, the underlying mechanism has yet to be fully elucidated. Here we show that the Arabidopsis ECHIDNA protein plays a role in flavonoid accumulation in the vacuole and protein trafficking to the vacuole. We found defective pigmentation patterns in echidna seed, possibly caused by reduced levels of proanthocyanidins, which determine seed coloration. The echidna mutant has defects in protein sorting to the protein storage vacuole as well as vacuole morphology. These findings indicate that ECHIDNA is involved in the vacuolar trafficking pathway as well as the previously described secretory pathway. In addition, we found a genetic interaction between echidna and green fluorescent seed 9 (gfs9), a membrane trafficking factor involved in flavonoid accumulation. Our findings suggest that vacuolar trafficking and/or vacuolar development, both of which are collectively regulated by ECHIDNA and GFS9, are required for flavonoid accumulation, resulting in seed coat pigmentation.
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