ArticleJournal of biological methods2020
An optimized procedure for isolation of rodent and human skeletal muscle sarcoplasmic and myofibrillar proteins.
Article in Journal of biological methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Effects of Peanut Protein Supplementation on Resistance Training Adaptations in Younger Adults.Nutrients · 2021Trial
- The effects of resistance training with or without peanut protein supplementation on skeletal muscle and strength adaptations in older individuals.Journal of the International Society of Sports Nutrition · 2020Trial
- Stable isotope-derived measurements of protein synthesis detect amino acid-induced anabolism in skeletal muscle microtissues not captured by puromycin labelling.Experimental physiology · 2026Article
- Aging concomitantly reduces skeletal muscle proteome plasticity and hypertrophic responses to resistance training in male adults.Physiological reports · 2026Article
- Skeletal muscle myosin heavy chain fragmentation following exercise may be linked to post-exercise inflammation and remodelling.Experimental physiology · 2026Article
- Article
- Ubiquitylomics: An Emerging Approach for Profiling Protein Ubiquitylation in Skeletal Muscle.Journal of cachexia, sarcopenia and muscle · 2024Review
- Skeletal muscle myosin heavy chain fragmentation as a potential marker of protein degradation in response to resistance training and disuse atrophy.Experimental physiology · 2024Article
- Targeted Bmal1 restoration in muscle prolongs lifespan with systemic health effects in aging model.JCI insight · 2024Article
- Skeletal muscle myosin heavy chain protein fragmentation as a potential marker of protein degradation in response to resistance training and disuse atrophy.bioRxiv : the preprint server for biology · 2024Article
- The effects of resistance training on denervated myofibers, senescent cells, and associated protein markers in middle-aged adults.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Article
- Mechanisms of mechanical overload-induced skeletal muscle hypertrophy: current understanding and future directions.Physiological reviews · 2023Review
- Skeletal Muscle Endogenous Estrogen Production Ameliorates the Metabolic Consequences of a High-Fat Diet in Male Mice.Endocrinology · 2023Article
- Molecular predictors of resistance training outcomes in young untrained female adults.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Metabolic reprogramming underlies cavefish muscular endurance despite loss of muscle mass and contractility.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Effects of High-Volume Versus High-Load Resistance Training on Skeletal Muscle Growth and Molecular Adaptations.Frontiers in physiology · 2022Article
- Frequent Manipulation of Resistance Training Variables Promotes Myofibrillar Spacing Changes in Resistance-Trained Individuals.Frontiers in physiology · 2021Article
- Myofibril and Mitochondrial Area Changes in Type I and II Fibers Following 10 Weeks of Resistance Training in Previously Untrained Men.Frontiers in physiology · 2021Article
- Identification of the MuRF1 Skeletal Muscle Ubiquitylome Through Quantitative Proteomics.Function (Oxford, England) · 2021Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several published protocols exist for isolating contractile or myofibrillar (MF) proteins from skeletal muscle, however, achieving complete resuspension of the myofibril pellet can be technically challenging. We performed several previously published MF isolation methods with the intent of determining which method was most suitable for MF protein isolation and solubilization. Here, we provide an optimized protocol to isolate sarcoplasmic and solubilized MF protein fractions from mammalian skeletal muscle suitable for several downstream assays.
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