ArticleJournal of molecular and cellular cardiology2020
Intact myocardial preparations reveal intrinsic transmural heterogeneity in cardiac mechanics.
Article in Journal of molecular and cellular cardiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 22 citations in OpenAlex.
- Cellular accumulation of lipofuscin in the heart: implications in health and disease.Histochemistry and cell biology · 2026Review
- Advances in Living Myocardial Slice Technology for Heart Failure Research.Current heart failure reports · 2026Review
- Review
- Living myocardial slices: walking the path towards standardization.Cardiovascular research · 2025Review
- Acute Biomechanical Effects of Cardiac Contractility Modulation in Living Myocardial Slices from End-Stage Heart Failure Patients.Bioengineering (Basel, Switzerland) · 2025Article
- IL-35 Ameliorates Myocardial Strain in Mice with T2DM-Induced Cardiac Injury: Assessment by Layer-Specific Strain.Diabetes, metabolic syndrome and obesity : targets and therapy · 2025Article
- MyoLoop: Design, development and validation of a standalone bioreactor for pathophysiological electromechanical in vitro cardiac studies.Experimental physiology · 2024Article
- Titin governs myocardial passive stiffness with major support from microtubules and actin and the extracellular matrix.Nature cardiovascular research · 2023Article
- Localized cardiac small molecule trajectories and persistent chemical sequelae in experimental Chagas disease.Nature communications · 2023Article
- Living myocardial slices: Advancing arrhythmia research.Frontiers in physiology · 2023Review
- A novel method to extend viability and functionality of living heart slices.Frontiers in cardiovascular medicine · 2023Article
- Animal models and animal-free innovations for cardiovascular research: current status and routes to be explored. Consensus document of the ESC Working Group on Myocardial Function and the ESC Working Group on Cellular Biology of the Heart.Cardiovascular research · 2022Article
- Mechanosensitive molecular mechanisms of myocardial fibrosis in living myocardial slices.ESC heart failure · 2022Article
- Remodelling of adult cardiac tissue subjected to physiological and pathological mechanical load in vitro.Cardiovascular research · 2022Article
- Sarcomere length in the beating heart: Synchronicity is optional.The Journal of general physiology · 2022Article
- Bottom-up proteomic analysis of human adult cardiac tissue and isolated cardiomyocytes.Journal of molecular and cellular cardiology · 2022Article
- Contractile State Dependent Sarcomere Length Variability in Isolated Guinea-Pig Cardiomyocytes.Frontiers in physiology · 2022Article
- Transcriptional bursts and heterogeneity among cardiomyocytes in hypertrophic cardiomyopathy.Frontiers in cardiovascular medicine · 2022Article
- TREK-1 in the heart: Potential physiological and pathophysiological roles.Frontiers in physiology · 2022Review
- Regional differences in arrhythmogenesis.The Journal of general physiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Determining transmural mechanical properties in the heart provides a foundation to understand physiological and pathophysiological cardiac mechanics. Although work on mechanical characterisation has begun in isolated cells and permeabilised samples, the mechanical profile of living individual cardiac layers has not been examined. Myocardial slices are 300 μm-thin sections of heart tissue with preserved cellular stoichiometry, extracellular matrix, and structural architecture. This allows for cardiac mechanics assays in the context of an intact in vitro organotypic preparation. In slices obtained from the subendocardium, midmyocardium and subepicardium of rats, a distinct pattern in transmural contractility is found that is different from that observed in other models. Slices from the epicardium and midmyocardium had a higher active tension and passive tension than the endocardium upon stretch. Differences in total myocyte area coverage, and aspect ratio between layers underlined the functional readouts, while no differences were found in total sarcomeric protein and phosphoprotein between layers. Such intrinsic heterogeneity may orchestrate the normal pumping of the heart in the presence of transmural strain and sarcomere length gradients in the in vivo heart.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.