Evidence map›Paper›PMID 32200874›Full record

ReviewThe Journal of investigative dermatology2020

Research Techniques Made Simple: CRISPR Genetic Screens.

Auke B C Otten, Bryan K Sun

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in The Journal of investigative dermatology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 25 citations in OpenAlex.

  1. Review
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  7. Elucidating Gene Functions in Congenital Heart Disease.Current treatment options in cardiovascular medicine · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Auke B C OttenDepartment of Dermatology, University of California San Diego, La Jolla, California, USA. Electronic address: aotten@ucsd.edu.
Bryan K SunDepartment of Dermatology, University of California San Diego, La Jolla, California, USA.
University of California San Diego · US

Funding

Secreted Regulators of Epidermal HomeostasisK08AR067853 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SUN, BRYAN · 2016 to 2020
$908k
CRISPR Screen to Identify Novel Secreted Epidermal RegulatorsR03AR075844 · NIAMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SUN, BRYAN · 2019 to 2020
$145k
Doris Duke Charitable Foundation 2019088NIAMS NIH HHS K08 AR067853NIAMS NIH HHS R03 AR075844
6 · The paper itself

Abstract

CRISPR and Cas proteins, often referred to as CRISPR/Cas, are the components of a bacterial genome editing system that can be used to perturb genes in cells and tissues. A classic application is to use CRISPR/Cas to generate genetic loss-of-function. When performed at large scale and combined with deep sequencing techniques, CRISPR-based perturbations can be performed in a high throughput setting to screen many candidate genomic elements for their roles in a phenotype of interest. Here, we discuss major considerations in the design, execution, and analysis of CRISPR screens. We focus on CRISPR knockout screens but also review adaptations to the CRISPR/Cas system that highlight the versatility of the system to make other types of experimental genetic changes as well. We also discuss examples of CRISPR genetic screens in investigative dermatology and how they may be used to answer key scientific questions in the field.

Indexed as

Research DesignClustered Regularly Interspaced Short Palindromic RepeatsGene EditingGenetic EngineeringGenetic TestingHumansPhenotype

Identifiers

PMID32200874
PMCPMC8525197
OpenAlexW3011343370

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.