Evidence map›Paper›PMID 32200762›Full record

ArticleJournal of biomedical science2020

Clinical-grade cryopreserved doxorubicin-loaded platelets: role of cancer cells and platelet extracellular vesicles activation loop.

Yu-Wen Wu, Cheng-Chain Huang, Chun Austin Changou, Long-Sheng Lu, Hadi Goubran, Thierry Burnouf

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 72 citations in OpenAlex.

  1. Review
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  4. Engineering platelets as cancer therapeutics.Nature reviews. Clinical oncology · 2026
    Review
  5. Article
  6. Natural carrier systems in cancer vaccines and immunotherapy.Human vaccines & immunotherapeutics · 2025
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Yu-Wen WuGraduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, 250 Wu-Xing Street, Taipei, 11031, Taiwan.ORCID https://orcid.org/0000-0001-7636-0393
Cheng-Chain HuangGraduate Institute of Translational Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Chun Austin ChangouGraduate Institute of Translational Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Long-Sheng LuGraduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, 250 Wu-Xing Street, Taipei, 11031, Taiwan.ORCID https://orcid.org/0000-0002-4247-1981
Hadi GoubranSaskatoon Cancer Centre and College of Medicine, University of Saskatchewan, Saskatchewan, Canada.
Thierry BurnoufGraduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, 250 Wu-Xing Street, Taipei, 11031, Taiwan. thburnouf@gmail.com.ORCID https://orcid.org/0000-0002-0507-9243
Taipei Medical University · TWTaipei Medical University Hospital · TWUniversity of Saskatchewan · CA

Funding

Ministry of Education Aim for the Top University PlanMinistry of Science and Technology, Taiwan 104-2314-B-038-040-MY3National Health Research Institute, Taiwan NHRI-EX109-10920EI
6 · The paper itself

Abstract

backgroundHuman platelets (PLT) and PLT-extracellular vesicles (PEV) released upon thrombin activation express receptors that interact with tumour cells and, thus, can serve as a delivery platform of anti-cancer agents. Drug-loaded nanoparticles coated with PLT membranes were demonstrated to have improved targeting efficiency to tumours, but remain impractical for clinical translation. PLT and PEV targeted drug delivery vehicles should facilitate clinical developments if clinical-grade procedures can be developed.

methodsPLT from therapeutic-grade PLT concentrate (PC; N > 50) were loaded with doxorubicin (DOX) and stored at - 80 °C (DOX-loaded PLT) with 6% dimethyl sulfoxide (cryopreserved DOX-loaded PLT). Surface markers and function of cryopreserved DOX-loaded PLT was confirmed by Western blot and thromboelastography, respectively. The morphology of fresh and cryopreserved naïve and DOX-loaded PLT was observed by scanning electron microscopy. The content of tissue factor-expressing cancer-derived extracellular vesicles (TF-EV) present in conditioned medium (CM) of breast cancer cells cultures was measured. The drug release by fresh and cryopreserved DOX-loaded PLT triggered by various pH and CM was determined by high performance liquid chromatography. The thrombin activated PEV was analyzed by nanoparticle tracking analysis. The cellular uptake of DOX from PLT was observed by deconvolution microscopy. The cytotoxicities of DOX-loaded PLT, cryopreserved DOX-loaded PLT, DOX and liposomal DOX on breast, lung and colon cancer cells were analyzed by CCK-8 assay.

results15~36 × 10

conclusionsCryopreserved DOX-loaded PLT can be prepared under clinically compliant conditions preserving the membrane functionality for anti-cancer therapy. These findings open perspectives for translational applications of PLT-based drug delivery systems.

Indexed as

CryopreservationBlood PlateletsDoxorubicinExtracellular VesiclesHumansNeoplastic Cells, CirculatingDoxorubicinCancerCryopreserved plateletDoxorubicinDrug deliveryPlatelet extracellular vesiclesTissue factor

Identifiers

PMID32200762
PMCPMC7087392
OpenAlexW3012757688

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.