ArticleGenome biology2020
Decode-seq: a practical approach to improve differential gene expression analysis.
Article in Genome biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Increasing usable reads in RNA-seq protocols.iScience · 2026Article
- Tatdn2 is required for DNA repair to safeguard genome stability in primordial germ cells.Nucleic acids research · 2025Article
- TIRE-seq simplifies transcriptomics via integrated RNA capture and library preparation.Scientific reports · 2025Article
- DeLTa-Seq: High-Throughput Targeted RNA-Seq of Rice Leaves Without RNA Purification.Methods in molecular biology (Clifton, N.J.) · 2025Article
- High-throughput transcriptional profiling of perturbations byJournal of pharmaceutical analysis · 2023Article
- Chromosome-level genome and high nitrogen stress response of the widespread and ecologically important wetland plantFrontiers in plant science · 2023Article
- 3' RNA-seq is superior to standard RNA-seq in cases of sparse data but inferior at identifying toxicity pathways in a model organism.Frontiers in bioinformatics · 2023Article
- Using Machine Learning to Identify Biomarkers Affecting Fat Deposition in Pigs by Integrating Multisource Transcriptome Information.Journal of agricultural and food chemistry · 2022Article
- Prime-seq, efficient and powerful bulk RNA sequencing.Genome biology · 2022Article
- Direct-TRI: High-throughput RNA-extracting Method for All Stages of Zebrafish Development.Bio-protocol · 2021Article
- Intracellular and Intercellular Gene Regulatory Network Inference From Time-Course Individual RNA-Seq.Frontiers in bioinformatics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many differential gene expression analyses are conducted with an inadequate number of biological replicates. We describe an easy and effective RNA-seq approach using molecular barcoding to enable profiling of a large number of replicates simultaneously. This approach significantly improves the performance of differential gene expression analysis. Using this approach in medaka (Oryzias latipes), we discover novel genes with sexually dimorphic expression and genes necessary for germ cell development. Our results also demonstrate why the common practice of using only three replicates in differential gene expression analysis should be abandoned.
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Registered trials
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