Evidence map›Paper›PMID 32198976›Full record

ArticleJournal of cellular and molecular medicine2020

Long non-coding RNA-H19 stimulates osteogenic differentiation of bone marrow mesenchymal stem cells via the microRNA-149/SDF-1 axis.

Guangjie Li, Xiangdong Yun, Kaishan Ye, Haiyan Zhao, Jiangdong An, Xueliang Zhang, Xingwen Han, Yanhong Li, Shuanke Wang

Expression of concernErratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
3.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.

  1. Pooled it
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  10. Long noncoding RNA (lncRNA)Genes & diseases · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Guangjie LiDepartment of Orthopedics, Lanzhou University Second Hospital, Lanzhou, China.
Xiangdong YunDepartment of Orthopedics, Lanzhou University Second Hospital, Lanzhou, China.
Kaishan YeDepartment of Orthopedics, Lanzhou University Second Hospital, Lanzhou, China.
Haiyan ZhaoThe First Hospital of Lanzhou University, Lanzhou, China.
Jiangdong AnDepartment of Orthopedics, Lanzhou University Second Hospital, Lanzhou, China.
Xueliang ZhangThe First Hospital of Lanzhou University, Lanzhou, China.
Xingwen HanThe First Hospital of Lanzhou University, Lanzhou, China.
Yanhong LiDepartment of Orthopedics, Lanzhou University Second Hospital, Lanzhou, China.
Shuanke WangDepartment of Orthopedics, Lanzhou University Second Hospital, Lanzhou, China.ORCID 0000-0002-0446-8327
Lanzhou University Second Hospital · CNFirst Hospital of Lanzhou University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone defects resulting from non-union fractures or tumour resections are common clinical problems. Long non-coding RNAs (lncRNAs) are reported to play vital roles in stem cell differentiation. The aim of this study was to elucidate the role of lncRNA-H19 in osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs). Following the establishment of an osteogenic differentiation model in rats, the expression of H19, microRNA-149 (miR-149) and stromal cell-derived factor-1 (SDF-1) was measured by RT-qPCR. Thereafter, BMMSCs were isolated from rats and treated with a series of mimic, inhibitor or siRNA. SDF-1 expression, alkaline phosphatase (ALP) activity and osteocalcin (OCN) content were detected. The mineralized and calcified nodules were assessed by alizarin red S and Von Kossa staining. BMMSC surface markers were detected by flow cytometry. Western blot analysis was used to measure the expression of ALP, OCN, runt-related transcription factor 2 (RUNX2) and osterix (OSX) proteins. Lastly, dual-luciferase reporter gene assay and RNA immunoprecipitation were applied to verify the relationship of H19, miR-149 and SDF-1. Overexpressed H19 and SDF-1 and poorly expressed miR-149 were found in rats with osteogenic differentiation. H19 increased SDF-1 expression by binding to miR-149. H19 enhanced ALP activity, OCN content, calcium deposit and ALP, OCN, RUNX2 and OSX protein expression of BMMSCS by up-regulating SDF-1 via binding to miR-149. Taken together, up-regulated H19 could promote the osteogenic differentiation of BMMSCs by increasing SDF-1 via miR-149.

Indexed as

Gene Expression RegulationAlkaline PhosphataseAnimalsBone Marrow CellsBone RegenerationCell DifferentiationChemokine CXCL12Core Binding Factor Alpha 1 SubunitGenes, ReporterMaleMesenchymal Stem CellsMicroRNAsOsteocalcinOsteogenesisRatsRats, Sprague-DawleyAlkaline PhosphataseChemokine CXCL12Core Binding Factor Alpha 1 SubunitCXCL12 protein, ratH19 long non-coding RNAMicroRNAsMIRN149 microRNA, ratOsteocalcinRNA, Long NoncodingRunx2 protein, ratSp7 protein, ratTranscription Factorsbone defectsbone marrow mesenchymal stem cellslong non-coding RNA-H19microRNA-149osteogenic differentiationstromal cell-derived factor-1

Identifiers

PMID32198976
PMCPMC7205807
OpenAlexW3012511087

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.