Evidence map›Paper›PMID 32198661›Full record

ArticleMikrochimica acta2020

A fluorescence signal amplification strategy for modification-free ratiometric determination of tyrosinase in situ based on the use of dual-templated copper nanoclusters.

Xuemin Huang, Huanan Zhao, Wen Qiu, Jian Wang, Longhua Guo, Zhenyu Lin, Wei Pan, Yong Wu, Bin Qiu

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Xuemin HuangMinistry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel farming and processing, Fuzhou University, Fuzhou, 350108, Fujian, China.
Huanan ZhaoMinistry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel farming and processing, Fuzhou University, Fuzhou, 350108, Fujian, China.
Wen QiuMinistry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel farming and processing, Fuzhou University, Fuzhou, 350108, Fujian, China.
Jian WangMinistry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel farming and processing, Fuzhou University, Fuzhou, 350108, Fujian, China.
Longhua GuoMinistry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel farming and processing, Fuzhou University, Fuzhou, 350108, Fujian, China.
Zhenyu LinMinistry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel farming and processing, Fuzhou University, Fuzhou, 350108, Fujian, China.
Wei PanInstitute of Agricultural Quality Standards and Testing Technology Research, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China. will-pan@sohu.com.
Yong WuXiamen Anxinya Biological Technology Co. Ltd, Xiamen, 361005, Fujian, China.
Bin QiuMinistry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, Eel farming and processing, Fuzhou University, Fuzhou, 350108, Fujian, China. summer328cn@163.com.
Fuzhou University · CNFujian Academy of Agricultural Sciences · CN

Funding

Fujian Provincial Department of Science and Technology 2018J01682Quality and Safety of Agricultural Products of Innovation Team of Fujian Academy of Agricultural Sciences STIT2017-1-12STS Key Project of Fujian Province 2017T3007
6 · The paper itself

Abstract

A fluorescence resonance energy transfer (FRET)-based in situ fluorescence signal amplification strategy is described for the determination of tyrosinase (TYR). In this assay, a dual-templated copper nanocluster (CuNCs) stabilized by bovine serum albumin (BSA) and glycylglycine (Gly-Gly) was used as an energy donor. Metyrosine was employed as a TYR substrate because its enzyme catalytic product (methyldopa) was able to function as a monomer molecule to form fluorescent polymethyldopa (PMeDP) with the assistance of BSA/Gly-Gly CuNCs. In this process, PMeDP can combine with BSA/Gly-Gly CuNCs without extra modification and then acts as an energy receptor, which leads to a remarkable FRET from BSA/Gly-Gly CuNCs to PMeDP. Interestingly, the fluorescence intensity of PMeDP was strengthened greatly in the FRET-based sensor compared to the separate excitation, which provided good sensitivity for TYR sensing. Illuminated under a UV light source, the fluorescence signal change is observed from dark violet to bright green. Therefore, the present sensing system affords a reliable ratiometric assay for TYR determination. Also, the ratio of fluorescence intensity between PMeDP (λ

Indexed as

CopperFluorescencealpha-MethyltyrosineFluorescence Resonance Energy TransferLimit of DetectionMetal NanoparticlesMethyldopaMonophenol Monooxygenasealpha-MethyltyrosineCopperMethyldopaMonophenol MonooxygenaseDual-templated copper nanoclustersFluorescence resonance energy transferRatiometric detectionTyrosinase

Identifiers

PMID32198661
OpenAlexW3011859564

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.