Evidence map›Paper›PMID 32198118›Full record

ArticleJournal of molecular biology2020

Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells.

Ziliang Huang, Mingxing Ouyang, Shaoying Lu, Yingxiao Wang, Qin Peng

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. A Perspective of Epigenetic Regulation in Radiotherapy.Frontiers in cell and developmental biology · 2021
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ziliang HuangDepartment of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Mingxing OuyangDepartment of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Shaoying LuDepartment of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Yingxiao WangDepartment of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: yiw015@eng.ucsd.edu.
Qin PengDepartment of Bioengineering, University of California, San Diego, La Jolla, CA 92093, USA; Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: q3peng@eng.ucsd.edu.
University of California, San Diego · US

Funding

Role of Spatiotemporal Epigenetic Dynamics in Regulating Endothelial Gene Expressions under FlowsR01HL121365 · NHLBI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHIEN, SHU, WANG, YINGXIAO · 2014 to 2025
$7.5M
Locus-specific Imaging of Dynamic Histone Methylations during ReprogrammingR01GM125379 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHIEN, SHU, WANG, YINGXIAO · 2017 to 2020
$2.4M
Integrators of Metastatic PotentialR01CA238042 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GHOSH, PRADIPTA, LUKER, GARY D · 2019 to 2023
$2.3M
Multiplex FRET Imaging of Kinase-Epigenome Interregulations in Live Cancer CellsR33CA204704 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WANG, YINGXIAO · 2017 to 2019
$1.1M
Remote-Control Mechano-Genetics and Epigenetics for Live Cell ManipulationR01GM126016 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WANG, YINGXIAO · 2018 to 2020
$1.1M
Engineering Machinery Molecules to Visualize and Actuate ImmunotherapyR21CA209629 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WANG, YINGXIAO · 2017 to 2018
$371k
NCI NIH HHS R01 CA238042NCI NIH HHS R21 CA209629NCI NIH HHS R33 CA204704NHLBI NIH HHS R01 HL121365NIGMS NIH HHS R01 GM125379NIGMS NIH HHS R01 GM126016
6 · The paper itself

Abstract

Previous studies with various Src family kinase biosensors showed that the nuclear kinase activities are much suppressed compared to those in the cytosol, suggesting that these kinases are regulated differently in the nucleus and in the cytosol. In this study, using Fyn as an example, we first engineered a Fyn biosensor with a light-inducible nuclear localization signal to demonstrate that the Fyn kinase activity is significantly lower in the nucleus than in the cytosol. To understand how different equilibrium states between Fyn and the corresponding phosphatases are maintained in the cytosol and nucleus, we further engineered a Fyn kinase domain with light-inducible nuclear localization signal. The results revealed that the Fyn kinase can be actively transported into the nucleus upon light activation and upregulate the biosensor signals in the nucleus. Our results suggest that there is limited transport or diffusion of Fyn kinase between the cytosol and nucleus in the cells, which is important for the maintenance of different equilibrium states of Fyn in situ.

Indexed as

OptogeneticsAnimalsBiosensing TechniquesCell NucleusCells, CulturedCytosolFibroblastsHEK293 CellsHumansNuclear Localization SignalsProtein TransportProto-Oncogene Proteins c-fynSubcellular FractionsNuclear Localization SignalsProto-Oncogene Proteins c-fynFRET biosensorFyn kinaselight-inducible kinaseLINuSlive cell imaging

Identifiers

PMID32198118
PMCPMC7225052
OpenAlexW3012472958

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.