ArticleBMC research notes2020
Improved detection of esp, hyl, asa1, gelE, cylA virulence genes among clinical isolates of Enterococci.
Article in BMC research notes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 71 citations in OpenAlex.
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- Extensively drug-resistant and heat-resistant Enterococcus faecalis and Enterococcus faecium in ready-to-eat meat products.NPJ science of food · 2026Article
- Vancomycin-resistant enterococci in healthcare settings: clonal analysis, resistance profiles, and biofilm formation of strains isolated from hospitalized patients.Folia microbiologica · 2026Article
- Oral microbiome contribution in colorectal carcinogenesis: polymicrobial interactions and virulence determinants.Frontiers in cellular and infection microbiology · 2026Review
- Molecular Profiling of CRISPR-Cas System, Virulence Traits, and Antimicrobial Resistance inInternational journal of microbiology · 2026Article
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- Genomic Characterization of Multiple Antibiotic-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- Characterization of Enterococcus species in surface drinking water from Akoko Edo Nigeria reveals contamination levels and risks to public health.Scientific reports · 2025Article
- Antimicrobial resistance and virulence potential of non-faecium and non-faecalis enterococci isolated from canine rectal swabs.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Genomic Epidemiology of Vancomycin-ResistantAntibiotics (Basel, Switzerland) · 2025Article
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- Molecular characterization of histidine and tyrosine decarboxylating Enterococcus species isolated from some milk products.BMC microbiology · 2025Article
- Novel strategies for vancomycin-resistant Enterococcus faecalis biofilm control: bacteriophage (vB_EfaS_ZC1), propolis, and their combined effects in an ex vivo endodontic model.Annals of clinical microbiology and antimicrobials · 2025Article
- Significant changes in gut microbiota and SCFAs among patients with newly diagnosed acute myeloid leukemia.Frontiers in microbiology · 2025Article
- Microbial dynamics of acute pancreatitis: integrating culture, sequencing, and bile impact on bacterial populations and gaseous metabolites.Frontiers in microbiology · 2025Article
- Beneficial and Safety Properties of a Bacteriocinogenic and Putative ProbioticFoods (Basel, Switzerland) · 2024Article
- Vancomycin resistance and virulence genes evaluation inHeliyon · 2024Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveVirulence factors (VFs) among the clinical strains of enterococci play a vital role in pathogenesis. This study was aimed to screen for cylA, asa1, gelE, esp and hyl among Enterococcus faecalis (n = 89) and E. faecium (n = 51) by multiplex PCR. The previously reported multiplex PCR was modified to 2 duplex (asa1 and gelE, cylA and esp) PCRs and 1 simplex (hyl) PCR. The idea of the modification of the multiplex PCR proposed here emerged in the course of the research study when majority of the isolates which phenotypically exhibited virulence traits were found to be negative for the respective gene.
resultscylA, gelE and asa1 were significantly predominant in E. faecalis (59.55%, 85.39%, 86.51%) than E. faecium (1.96%, 60.78%, 9.80%) (p < 0.0001, p = 0.001967, p < 0.0001). hyl was detected in E. faecium (5.9%) only. The number of VFs detected in each isolate was recorded as the VF score. E. faecalis isolates had a VF score pattern of score 4 (34.83%), score 3 (26.96%), score 2 (28.08%) and score 1 (8.98%) while E. faecium had score 4 (1.96%), score 3 (7.84%), score 2 (25.49%) and score 1 (41.18%). This modification of the PCR protocol could resolve the problem of decreased detection of virulence determinants in enterococci.
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