ArticleMolecular plant pathology2020
Reduction of Phakopsora pachyrhizi infection on soybean through host- and spray-induced gene silencing.
Article in Molecular plant pathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 78 citations in OpenAlex.
- Nano-enabled spray-induced RNA interference against fungal pathogens of oilseed crops: carrier performance, delivery evidence and field translation.World journal of microbiology & biotechnology · 2026Review
- Screening Various Bacterial-Produced Double-Stranded RNAs for Managing Asian Soybean Rust Disease Caused byPlants (Basel, Switzerland) · 2026Article
- A novel self-assembled nanocarrier-mediated dsRNA fungicide for broad-spectrum management ofMaterials today. Bio · 2025Article
- Harnessing double-stranded RNA (dsRNA): a sustainable approach to pest management.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Regulation of Plant Genes with Exogenous RNAs.International journal of molecular sciences · 2025Review
- Trans-Kingdom sRNA Silencing inPathogens (Basel, Switzerland) · 2025Review
- Article
- Integrating RNA Interference and Nanotechnology: A Transformative Approach in Plant Protection.Plants (Basel, Switzerland) · 2025Review
- Review
- Decoding the biochemical dialogue: metabolomic insights into soybean defense strategies against diverse pathogens.Science China. Life sciences · 2024Article
- Exogenous Application of dsRNA in Plant Protection: Efficiency, Safety Concerns and Risk Assessment.International journal of molecular sciences · 2024Review
- Control of Sclerotinia sclerotiorum via an RNA interference (RNAi)-mediated targeting of SsPac1 and SsSmk1.Planta · 2024Article
- Identification of self- and pathogen-targeted miRNAs from resistant and susceptible Theobroma cacao variety to black pod disease.Scientific reports · 2024Article
- Soybean-Phakopsora pachyrhizi interactions: towards the development of next-generation disease-resistant plants.Plant biotechnology journal · 2024Review
- Double-stranded RNA prevents and cures infection by rust fungi.Communications biology · 2023Article
- Improving RNA-based crop protection through nanotechnology and insights from cross-kingdom RNA trafficking.Current opinion in plant biology · 2023Review
- Why Do We Need Alternative Methods for Fungal Disease Management in Plants?Plants (Basel, Switzerland) · 2023Review
- Spray-induced gene silencing to identify powdery mildew gene targets and processes for powdery mildew control.Molecular plant pathology · 2023Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Foxtail mosaic virus: A tool for gene function analysis in maize and other monocots.Molecular plant pathology · 2023Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Asian soybean rust (ASR), caused by the obligate fungal pathogen Phakopsora pachyrhizi, often leads to significant yield losses and can only be managed through fungicide applications currently. In the present study, eight urediniospore germination or appressorium formation induced P. pachyrhizi genes were investigated for their feasibility to suppress ASR through a bean pod mottle virus (BPMV)-based host-induced gene silencing (HIGS) strategy. Soybean plants expressing three of these modified BPMV vectors suppressed the expression of their corresponding target gene by 45%-80%, fungal biomass accumulation by 58%-80%, and significantly reduced ASR symptom development in soybean leaves after the plants were inoculated with P. pachyrhizi, demonstrating that HIGS can be used to manage ASR. In addition, when the in vitro synthesized double-stranded RNAs (dsRNAs) for three of the genes encoding an acetyl-CoA acyltransferase, a 40S ribosomal protein S16, and glycine cleavage system H protein were sprayed directly onto detached soybean leaves prior to P. pachyrhizi inoculation, they also resulted in an average of over 73% reduction of pustule numbers and 75% reduction in P. pachyrhizi biomass accumulation on the detached leaves compared to the controls. To the best of our knowledge, this is the first report of suppressing P. pachyrhizi infection in soybean through both HIGS and spray-induced gene silencing. It was demonstrated that either HIGS constructs targeting P. pachyrhizi genes or direct dsRNA spray application could be an effective strategy for reducing ASR development on soybean.
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