Evidence map›Paper›PMID 32196803›Full record

ArticleAmerican journal of reproductive immunology (New York, N.Y. : 1989)2020

Pregnancy associates with alterations to the host and microbial proteome in vaginal mucosa.

Christina Farr Zuend, Nicole H Tobin, Trisha Vera, Lani Kotyrba, Laura Noël-Romas, Kenzie Birse, Sarah Mutch, Fan Li, David Lee, Stuart McCorrister and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of reproductive immunology (New York, N.Y. : 1989), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Interactions between microbiota and cervical epithelial, immune, and mucus barrier.Frontiers in cellular and infection microbiology · 2023
    Review
  5. Review
  6. Pregnancy associates with alterations to the host and microbial proteome in vaginal mucosa.American journal of reproductive immunology (New York, N.Y. : 1989) · 2020
    Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 3 countries.

Christina Farr ZuendNational HIV and Retrovirology Labs, J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada.
Nicole H TobinDepartment of Pediatrics, University of California, Los Angeles, Los Angeles, CA, USA.
Trisha VeraNational HIV and Retrovirology Labs, J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada.
Lani KotyrbaNational HIV and Retrovirology Labs, J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada.
Laura Noël-RomasNational HIV and Retrovirology Labs, J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada.
Kenzie BirseNational HIV and Retrovirology Labs, J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada.
Sarah MutchNational HIV and Retrovirology Labs, J.C. Wilt Infectious Diseases Research Centre, Public Health Agency of Canada, Winnipeg, MB, Canada.
Fan LiDepartment of Pediatrics, University of California, Los Angeles, Los Angeles, CA, USA.
David LeeDepartment of Pediatrics, University of California, Los Angeles, Los Angeles, CA, USA.
Stuart McCorristerMass Spectrometry and Proteomics Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Garrett WestmacottMass Spectrometry and Proteomics Core Facility, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
Grace M AldrovandiDepartment of Pediatrics, University of California, Los Angeles, Los Angeles, CA, USA.
Adam D BurgenerDepartment of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada.ORCID 0000-0002-2303-5738
Public Health Agency of Canada · CAUniversity of California, Los Angeles · USKarolinska Institutet · SE

Funding

LOC-IMPAACT Leadership GroupPharmacokinetics and Safety of Remdesivir for Treatment of COVID-19 in Pregnant Women in the USUM1AI068632 · NIAID · SOCIAL AND SCIENTIFIC SYSTEMS, INC. · PI Sharon A Nachman · 2011 to 2026
$278.1M
Statistical and Data Management Center-Pediatric,Adolescent, and Maternal CTGUM1AI068616 · NIAID · HARVARD UNIVERSITY D/B/A HARVARD SCHOOL OF PUBLIC HEALTH · PI Sean Scott Brummel, Marlene Ann Cooper · 2011 to 2026
$155.6M
LC - IMPAACT Leadership GroupUM1AI106716 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Grace M Aldrovandi · 2014 to 2026
$47.2M
CIHR TMI-138658NIAID NIH HHS UM1 AI068616NIAID NIH HHS UM1 AI068632NIAID NIH HHS UM1 AI106716NIH HHS 5R01AI28796NIH HHS UM1AI068632NIH HHS UM1AI106716
6 · The paper itself

Abstract

problemPregnant women are at increased risk of HIV acquisition, but the biological mechanisms contributing to this observation are not well understood. METHOD OF STUDY: Here, we assessed host immune and microbiome differences in the vaginal mucosa of healthy pregnant and non-pregnant women using a metaproteomics approach. Cervicovaginal lavage (CVL) samples were collected from 23 pregnant and 25 non-pregnant women.

resultsMass spectrometry analysis of CVL identified 550 human proteins and 376 bacterial proteins from 11 genera. Host proteome analysis indicated 56 human proteins (10%) were differentially abundant (P < .05) between pregnant and non-pregnant women, including proteins involved in angiogenesis (P = 3.36E-3), cell movement of phagocytes (P = 1.34E-6), and permeability of blood vessels (P = 1.27E-4). The major bacterial genera identified were Lactobacillus, Gardnerella, Prevotella, Megasphaera, and Atopobium. Pregnant women had higher levels of Lactobacillus species (P = .017) compared with non-pregnant women. Functional pathway analysis indicated that pregnancy associated with changes to bacterial metabolic pathway involved in energy metabolism, which were increased in pregnant women (P = .035).

conclusionOverall, pregnant women showed differences in the cervicovaginal proteome and microbiome that may be important for HIV infection risk.

Indexed as

PregnancyAdolescentAdultEnergy MetabolismFemaleHumansLactobacillusMass SpectrometryMicrobiotaMiddle AgedMucous MembraneProteomeVaginaYoung AdultProteomeHIVmicrobiomepregnancyproteomics

Identifiers

PMID32196803
PMCPMC7317380
OpenAlexW3010771057

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.