ArticleScience advances2020
Multiplexed single-molecule enzyme activity analysis for counting disease-related proteins in biological samples.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 68 citations in OpenAlex.
- Chemical basis of fluorogenic substrate probe design: revisiting the past to discover the new in biochemistry.RSC chemical biology · 2026Review
- Synthesis Based on Covalent Capture and Release Enables Purification-free Fluorogenic Probe Libraries for Single-Molecule Protease Activity Profiling.ACS central science · 2026Article
- Development of Single-Molecule Enzyme Activity Assay for Serine Hydrolases Using Activity-Based Protein Labeling Probes.Small methods · 2025Article
- Digital SERS bioanalysis of single-enzyme biomarkers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Functional Raman Probes for Detecting Enzyme Activities Based on Aggregation Control.Analytical chemistry · 2025Article
- Single-Molecule Enzyme Activity Analysis for Illuminating Pathological Proteoforms.ACS central science · 2025Review
- Thymidine Phosphodiester Chemiluminescent Probe for Sensitive and Selective Detection of Ectonucleotide Pyrophosphatase 1.Bioconjugate chemistry · 2025Article
- Hydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes.iScience · 2024Article
- Compact Digital Immunoassay Platform Integrating ELISA with a Lateral Flow Strip.Biomedicines · 2024Article
- Portable wide-field femtoliter-chamber imaging system for point-of-care digital bioanalysis.iScience · 2024Article
- ifDEEPre: large protein language-based deep learning enables interpretable and fast predictions of enzyme commission numbers.Briefings in bioinformatics · 2024Article
- Exploring fluoropolymers for fabrication of femtoliter chamber arrays used in digital bioanalysis.Scientific reports · 2024Article
- Thioester-Based Coupled Fluorogenic Assays in Microdevice for the Detection of Single-Molecule Enzyme Activities of Esterases with Specified Substrate Recognition.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Identification of activity-based biomarkers for early-stage pancreatic tumors in blood using single-molecule enzyme activity screening.Cell reports methods · 2024Article
- Toward versatile digital bioanalysis.Biomicrofluidics · 2023Article
- Recent methodological advances towards single-cell proteomics.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2023Review
- Digital Cascade Assays for ADP- or ATP-Producing Enzymes Using a Femtoliter Reactor Array Device.ACS sensors · 2023Article
- Induced-volatolomics for the design of tumour activated therapy.Chemical science · 2023Article
- Development of fluorogenic substrates for colorectal tumor-related neuropeptidases for activity-based diagnosis.Chemical science · 2023Article
- Emerging platforms for high-throughput enzymatic bioassays.Trends in biotechnology · 2023Review
Corrections and comments
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Authors and funding
12 authors at 8 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We established an ultrasensitive method for identifying multiple enzymes in biological samples by using a multiplexed microdevice-based single-molecule enzymatic assay. We used a paradigm in which we "count" the number of enzyme molecules by profiling their single enzyme activity characteristics toward multiple substrates. In this proof-of-concept study of the single enzyme activity-based protein profiling (SEAP), we were able to detect the activities of various phosphoric ester-hydrolyzing enzymes such as alkaline phosphatases, tyrosine phosphatases, and ectonucleotide pyrophosphatases in blood samples at the single-molecule level and in a subtype-discriminating manner, demonstrating its potential usefulness for the diagnosis of diseases based on ultrasensitive detection of enzymes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.