ArticleScience advances2020
An array of 60,000 antibodies for proteome-scale antibody generation and target discovery.
Article in Science advances, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Discovery and Optimization of AMT-676, a CDH17-Targeting ADC for the Treatment of Advanced Gastrointestinal Cancers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Novel FLI1 Monoclonal Antibody Which Recognizes EWS::FLI1 with High Affinity Is Useful for Detecting Ewing Sarcoma.Antibodies (Basel, Switzerland) · 2025Article
- Development of antibody drug conjugates targeting epithelial membrane protein 2-highly expressed lung cancer.Cell death & disease · 2025Article
- Protein Microarray Analysis with GenePix.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cell-autonomous targeting of arabinogalactan by host immune factors inhibits mycobacterial growth.eLife · 2024Article
- Antibody array-based proteome approach reveals proteins involved in grape seed development.Plant physiology · 2024Article
- The PPO family in Nicotiana tabacum is an important regulator to participate in pollination.BMC plant biology · 2024Article
- PhIP-Seq: methods, applications and challenges.Frontiers in bioinformatics · 2024Review
- Protein Lactylation and Metabolic Regulation of the Zoonotic Parasite Toxoplasma gondii.Genomics, proteomics & bioinformatics · 2023Article
- A Cell Surface-Binding Antibody Atlas Nominates a MUC18-Directed Antibody-Drug Conjugate for Targeting Melanoma.Cancer research · 2023Article
- Acetylation coordinates the crosstalk between carbon metabolism and ammonium assimilation in Salmonella enterica.The EMBO journal · 2023Article
- A Proteomics Insight into Advancements in the Rice-Microbe Interaction.Plants (Basel, Switzerland) · 2023Review
- High-throughput membrane-anchored proteome screening reveals PIEZO1 as a promising antibody-drug target for human esophageal squamous cell carcinoma.Cancer medicine · 2022Article
- Retrospective analysis of the preparation and application of immunotherapy in cancer treatment (Review).International journal of oncology · 2022Article
- Circulating TGF-β Pathway in Osteogenesis Imperfecta Pediatric Patients Subjected to MSCs-Based Cell Therapy.Frontiers in cell and developmental biology · 2022Article
- Linear epitope landscape of the SARS-CoV-2 Spike protein constructed from 1,051 COVID-19 patients.Cell reports · 2021Article
- Antigen discovery tools for adaptive immune receptor repertoire research.Current opinion in systems biology · 2020Article
- Proteomics in Non-model Organisms: A New Analytical Frontier.Journal of proteome research · 2020Article
- TJP1, a Membrane-Expressed Protein, is a Potential Therapeutic and Prognostic Target for Lung Cancer.Technology in cancer research & treatmentArticle
Corrections and comments
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Authors and funding
43 authors at 13 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibodies are essential for elucidating gene function. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 monoclonal antibodies (mAbs) against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic antibody feature. Distinctive combinations of 10,000 to 20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-protein pairs were found for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for membrane proteins and chromatin immunoprecipitation-sequencing mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues identified internalizing tumor antigens for antibody-drug conjugates. By finding high-affinity mAbs at a fraction of current time and cost, PETAL enables proteome-scale antibody generation and target discovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.