Evidence map›Paper›PMID 32195288›Full record

ArticleIranian journal of biotechnology2019

Evaluation of Immunogenicity, Protective Immunity on Aquaculture Pathogenic

Chen Chen, Chao Kang, Na Rong, Nana Wu, Chunlin Chen, Sanqiao Wu, Xiaoying Zhang, Xiang Liu

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Iranian journal of biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Chen ChenChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Chao KangChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Na RongChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Nana WuChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Chunlin ChenChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Sanqiao WuChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Xiaoying ZhangChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Xiang LiuChinese-German joint Institute for natural product research / Shaanxi Engineering Research Center for Tall Gastrodia Tuber and Medical Dogwood / College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, China.
Shaanxi University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

background

objectivesWe aimed to evaluate the immunogenicity, protective immunity, and prokaryotic expression fermentation of MATERIAL AND

methodsA molecular cloning method was used to construct the expression strain of FlaC protein, and the protein was purified with Ni-affinity chromatography. Polyclonal antiserum was prepared via mice immunized with the FlaC protein. The Western blot and enzyme-linked immunosorbent assay (ELISA) were used to check the specificity and titre of the antiserum. ELISA and pull-down assay detected the interaction between FlaC protein antiserum and

resultsThe expression strain of FlaC protein was obtained successfully, and purified FlaC protein was prepared using a mice polyclonal antibody. The FlaC protein antiserum held a high specificity, and the titre was 13200. The antiserum directly interacted with

conclusionsThis study showed that the FlaC protein possesses a significant immunogenicity and immune protection effect and obtained the optimal fermentation conditions. It is expected to be a potential vaccine against

Indexed as

FlaJ ProteinVibrio alginolyticusVibrio parahaemolyticus

Identifiers

PMID32195288
PMCPMC7080974
OpenAlexW2987632080

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.