ArticleHistochemistry and cell biology2020
Nanoscale imaging using differential expansion microscopy.
Article in Histochemistry and cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures.The Journal of cell biology · 2025Article
- Microscopy methods for the in vivo study of nanoscale nuclear organization.Biochemical Society transactions · 2025Review
- Accelerated protein retention expansion microscopy using microwave radiation.Cell reports methods · 2024Article
- Ultrastructure expansion microscopy (U-ExM) of mouse and human kidneys for analysis of subcellular structures.Cytoskeleton (Hoboken, N.J.) · 2024Article
- One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology.bioRxiv : the preprint server for biology · 2024Article
- EDTP enhances and protects the fluorescent signal of GFP in cleared and expanded tissues.Scientific reports · 2024Article
- Accelerated protein retention expansion microscopy using microwave radiation.bioRxiv : the preprint server for biology · 2024Article
- Toward a comprehensive view of gene architecture during transcription.Current opinion in genetics & development · 2024Review
- Combining single-molecule and expansion microscopy in fission yeast to visualize protein structures at the nanostructural level.Open biology · 2024Article
- Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin.Science (New York, N.Y.) · 2023Article
- Imaging nanoscale nuclear structures with expansion microscopy.Journal of cell science · 2022Article
- Cell3: a new vision for study of the endomembrane system in mammalian cells.Bioscience reports · 2021Review
- Expansion microscopy facilitates quantitative super-resolution studies of cytoskeletal structures in kinetoplastid parasites.Open biology · 2021Article
- Protein-retention expansion microscopy for visualizing subcellular organelles in fixed brain tissue.Journal of neuroscience methods · 2021Article
- EphrinB2 and GRIP1 stabilize mushroom spines during denervation-induced homeostatic plasticity.Cell reports · 2021Article
- Challenges of Using Expansion Microscopy for Super-resolved Imaging of Cellular Organelles.Chembiochem : a European journal of chemical biology · 2021Article
- Prospects and limitations of expansion microscopy in chromatin ultrastructure determination.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Article
- Practical method for superresolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification.Molecular biology of the cell · 2020Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Expensive and time-consuming approaches of immunoelectron microscopy of biopsy tissues continues to serve as the gold-standard for diagnostic pathology. The recent development of the new approach of expansion microscopy (ExM) capable of fourfold lateral expansion of biological specimens for their morphological examination at approximately 70 nm lateral resolution using ordinary diffraction limited optical microscopy, is a major advancement in cellular imaging. Here we report (1) an optimized fixation protocol for retention of cellular morphology while obtaining optimal expansion, (2) an ExM procedure for up to eightfold lateral and over 500-fold volumetric expansion, (3) demonstrate that ExM is anisotropic or differential between tissues, cellular organelles and domains within organelles themselves, and (4) apply image analysis and machine learning (ML) approaches to precisely assess differentially expanded cellular structures. We refer to this enhanced ExM approach combined with ML as differential expansion microscopy (DiExM), applicable to profiling biological specimens at the nanometer scale. DiExM holds great promise for the precise, rapid and inexpensive diagnosis of disease from pathological specimen slides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.