Evidence map›Paper›PMID 32192526›Full record

ArticleJournal of neuroinflammation2020

Metastatic breast cancer cells induce altered microglial morphology and electrical excitability in vivo.

Anna Simon, Ming Yang, Joanne L Marrison, Andrew D James, Mark J Hunt, Peter J O'Toole, Paul M Kaye, Miles A Whittington, Sangeeta Chawla, William J Brackenbury

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 27 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Microglia and metastases to the central nervous system: victim, ravager, or something else?Journal of experimental & clinical cancer research : CR · 2022
    Review
  10. Review
  11. Targeting Ion Channels for Cancer Treatment: Current Progress and Future Challenges.Reviews of physiology, biochemistry and pharmacology · 2022
    Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Anna SimonDepartment of Biology, University of York, Heslington, York, YO10 5DD, UK.
Ming YangDepartment of Biology, University of York, Heslington, York, YO10 5DD, UK.
Joanne L MarrisonBioscience Technology Facility, Department of Biology, University of York, Heslington, York, YO10 5DD, UK.
Andrew D JamesDepartment of Biology, University of York, Heslington, York, YO10 5DD, UK.
Mark J HuntHull York Medical School, Heslington, York, YO10 5DD, UK.
Peter J O'TooleBioscience Technology Facility, Department of Biology, University of York, Heslington, York, YO10 5DD, UK.
Paul M KayeDepartment of Biology, University of York, Heslington, York, YO10 5DD, UK.
Miles A WhittingtonHull York Medical School, Heslington, York, YO10 5DD, UK.
Sangeeta ChawlaDepartment of Biology, University of York, Heslington, York, YO10 5DD, UK. Sangeeta.chawla@york.ac.uk.
William J BrackenburyDepartment of Biology, University of York, Heslington, York, YO10 5DD, UK. william.brackenbury@york.ac.uk.ORCID http://orcid.org/0000-0001-6882-3351
University of York · GBHull York Medical School · GB

Funding

Cancer Research UK 25922Cancer Research UK 30380Cancer Research UK A25922Medical Research Council G1000508Wellcome TrustWellcome Trust Centre for Chronic Diseases and Disorders (C2D2) and Centre for Future Health (CFH)
6 · The paper itself

Abstract

backgroundAn emerging problem in the treatment of breast cancer is the increasing incidence of metastases to the brain. Metastatic brain tumours are incurable and can cause epileptic seizures and cognitive impairment, so better understanding of this niche, and the cellular mechanisms, is urgently required. Microglia are the resident brain macrophage population, becoming "activated" by neuronal injury, eliciting an inflammatory response. Microglia promote proliferation, angiogenesis and invasion in brain tumours and metastases. However, the mechanisms underlying microglial involvement appear complex and better models are required to improve understanding of function.

methodsHere, we sought to address this need by developing a model to study metastatic breast cancer cell-microglial interactions using intravital imaging combined with ex vivo electrophysiology. We implanted an optical window on the parietal bone to facilitate observation of cellular behaviour in situ in the outer cortex of heterozygous Cx3cr1

resultsWe detected GFP-expressing microglia in Cx3cr1

conclusionsThese data suggest that peritumoral microglial activation and accumulation may play a critical role in local tissue changes underpinning aberrant cortical activity, which offers a possible mechanism for the disrupted cognitive performance and seizures seen in patients with metastatic breast cancer.

Indexed as

Disease Models, AnimalMicrogliaAnimalsBrain NeoplasmsBreast NeoplasmsCell Line, TumorFemaleIntravital MicroscopyMiceMice, Inbred C57BLMice, TransgenicTumor MicroenvironmentBreast cancerElectrophysiologyIntravital imagingMetastasisMicroglia

Identifiers

PMID32192526
PMCPMC7081703
OpenAlexW3012725666

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.