Evidence map›Paper›PMID 32192516›Full record

ArticleMicrobial cell factories2020

Identification and engineering of 32 membered antifungal macrolactone notonesomycins.

Falicia Goh, Mingzi M Zhang, Tian Ru Lim, Kia Ngee Low, Choy Eng Nge, Elena Heng, Wan Lin Yeo, Fernanda L Sirota, Sharon Crasta, Zann Tan and 11 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Application of Cas12j forBiomolecules · 2024
    Article
  3. Article
  4. Antibacterial Spirotetronate Polyketides from anMolecules (Basel, Switzerland) · 2022
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Falicia GohBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Mingzi M ZhangMetabolic Engineering, Functional Molecules & Polymers, Institute of Chemical and Engineering Sciences, A*STAR, 31 Biopolis Way, Nanos #01-01, Singapore, 138669, Singapore.
Tian Ru LimBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Kia Ngee LowBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Choy Eng NgeBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Elena HengMolecular Engineering Laboratory, Institute of Bioengineering and Nanotechnology, A*STAR, 31 Biopolis Way, Nanos, Singapore, 138669, Singapore.
Wan Lin YeoMetabolic Engineering, Functional Molecules & Polymers, Institute of Chemical and Engineering Sciences, A*STAR, 31 Biopolis Way, Nanos #01-01, Singapore, 138669, Singapore.
Fernanda L SirotaBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Sharon CrastaBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Zann TanBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Veronica NgBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Chung Yan LeongBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Huibin ZhangGenome Institute of Singapore, A*STAR, 60 Biopolis Street, Genome #02-01, Singapore, 138672, Singapore.
Alexander LezhavaGenome Institute of Singapore, A*STAR, 60 Biopolis Street, Genome #02-01, Singapore, 138672, Singapore.
Swaine L ChenGenome Institute of Singapore, A*STAR, 60 Biopolis Street, Genome #02-01, Singapore, 138672, Singapore.
Shawn S HoonMolecular Engineering Laboratory, Institute of Bioengineering and Nanotechnology, A*STAR, 31 Biopolis Way, Nanos, Singapore, 138669, Singapore.
Frank EisenhaberBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Birgit EisenhaberBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Yoganathan KanagasundaramBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore.
Fong T WongMolecular Engineering Laboratory, Institute of Bioengineering and Nanotechnology, A*STAR, 31 Biopolis Way, Nanos, Singapore, 138669, Singapore. wongft@ibn.a-star.edu.sg.
Siew Bee NgBioinformatics Institute, A*STAR, 30 Biopolis Street, #07-01 Matrix, Singapore, 138671, Singapore. ngsb@bii.a-star.edu.sg.

Funding

National Research Foundation Singapore NRF2013-THE001-094
6 · The paper itself

Abstract

Notonesomycin A is a 32-membered bioactive glycosylated macrolactone known to be produced by Streptomyces aminophilus subsp. notonesogenes 647-AV1 and S. aminophilus DSM 40186. In a high throughput antifungal screening campaign, we identified an alternative notonesomycin A producing strain, Streptomyces sp. A793, and its biosynthetic gene cluster. From this strain, we further characterized a new more potent antifungal non-sulfated analogue, named notonesomycin B. Through CRISPR-Cas9 engineering of the biosynthetic gene cluster, we were able to increase the production yield of notonesomycin B by up to 18-fold as well as generate a strain that exclusively produces this analogue.

Indexed as

Antifungal AgentsCloning, MolecularMacrolidesMultigene FamilyStreptomycesAntifungal AgentsMacrolidesnotonesomycin ABiosynthetic gene clusterCRISPR–Cas9Natural productPolyketideStreptomyces antibiotic regulatory proteinSulfation

Identifiers

PMID32192516
PMCPMC7081687

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.