ArticleChemical science2019
Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Combinatorial Optimization of Protein Systems in Synthetic Cells.ACS synthetic biology · 2026Article
- Cell-Size Confinement Drives Size-Dependent Scaling of Intracellular Reaction-Diffusion Waves for Robust Patterning.Small science · 2026Article
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Cell-Free Protein Expression by a Reconstituted Transcription-Translation System Energized by Sugar Catabolism.Molecules (Basel, Switzerland) · 2024Article
- Machine learning-aided design and screening of an emergent protein function in synthetic cells.Nature communications · 2024Article
- Multimolecular Competition Effect as a Modulator of Protein Localization and Biochemical Networks in Cell-Size Space.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Insights into the assembly and regulation of the bacterial divisome.Nature reviews. Microbiology · 2024Review
- Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves.Biophysics and physicobiology · 2024Review
- Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide.Journal of nanobiotechnology · 2022Article
- In vitro assembly, positioning and contraction of a division ring in minimal cells.Nature communications · 2022Article
- Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells.Science advances · 2022Article
- Capsule-like DNA Hydrogels with Patterns Formed by Lateral Phase Separation of DNA Nanostructures.JACS Au · 2022Article
- Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.Frontiers in bioengineering and biotechnology · 2022Article
- Activation of a dilutedSTAR protocols · 2021Article
- System concentration shift as a regulator of transcription-translation system within liposomes.iScience · 2021Article
- A Relationship between NTP and Cell Extract Concentration for Cell-Free Protein Expression.Life (Basel, Switzerland) · 2021Article
- Navigating Multi-Scale Cancer Systems Biology Towards Model-Driven Clinical Oncology and Its Applications in Personalized Therapeutics.Frontiers in oncology · 2021Review
- Self-organization Assay for Min Proteins ofBio-protocol · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatiotemporal patterning is a fundamental mechanism for developmental differentiation and homeostasis in living cells. Because spatiotemporal patterns are based on higher-order collective motions of elements synthesized from genes, their behavior dynamically changes according to the element amounts. Thus, to understand life and use this process for material application, creation of artificial cells with time development of spatiotemporal patterning by changes of element levels is necessary. However, realizing coupling between spatiotemporal patterning and synthesis of elements in artificial cells has been particularly challenging. In this study, we established a system that can synthesize a patterning mechanism of the bacterial cell division plane (the so-called Min system) in artificial cells by modifying a defined protein expression system and demonstrated that artificial cells can show time development of spatiotemporal patterning similar to living cells. This system also allows generation and disappearance of spatiotemporal patterning, is controllable by a small molecule in artificial cells, and has the ability for application in cargo transporters. The system developed here provides a new material and a technique for understanding life, development of drug delivery tools, and creation of molecular robots.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.