ArticlePlant methods2020
A multiplex guide RNA expression system and its efficacy for plant genome engineering.
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 72 citations in OpenAlex.
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- CRISPR/Cas9 editing of β-Conglycinin subunits reduces IgE binding in soybean [Glycine max (L.) Merr.].BMC plant biology · 2026Article
- MYC2 and MYC3 orchestrate pith lignification to defend Nicotiana attenuata stems against a stem-boring weevil.The New phytologist · 2025Article
- CRISPR/Cas9-mediated simultaneous targeting of GmP34 and its homologs produces T-DNA-free soybean mutants with reduced allergenic potential.Frontiers in plant science · 2025Article
- Development of super-infective ternary vector systems for enhancing theHorticulture research · 2024Article
- CRISPR-Cas9/Cas12a systems for efficient genome editing and large genomic fragment deletions inFrontiers in bioengineering and biotechnology · 2024Article
- Engineering homoeologs provide a fine scale for quantitative traits in polyploid.Plant biotechnology journal · 2023Article
- OptimisedGenes · 2023Article
- CRISPR/Cas9-mediated gene editing to confer turnip mosaic virus (TuMV) resistance in Chinese cabbage (Horticulture research · 2023Article
- In Vivo Rapid Investigation of CRISPR-Based Base Editing Components inInternational journal of molecular sciences · 2022Article
- RPS5A Promoter-Driven Cas9 Produces Heritable Virus-Induced Genome Editing inMolecules and cells · 2021Article
- Construction of Multiple Guide RNAs in CRISPR/Cas9 Vector Using Stepwise or Simultaneous Golden Gate Cloning: Case Study for Targeting thePlants (Basel, Switzerland) · 2021Article
- The GB4.0 Platform, an All-In-One Tool for CRISPR/Cas-Based Multiplex Genome Engineering in Plants.Frontiers in plant science · 2021Article
- Applications and Major Achievements of Genome Editing in Vegetable Crops: A Review.Frontiers in plant science · 2021Review
- Development of a Highly Efficient Multiplex Genome Editing System in Outcrossing Tetraploid Alfalfa (Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe
resultsWe introduce a PCR-free multiple gRNA cloning system for editing plant genomes. This method consists of two steps: (1) cloning the annealed products of two single-stranded oligonucleotide fragments harboring a complimentary target-binding sequence on each strand between tRNA and gRNA scaffold sequences in a pGRNA vector; and (2) assembling tRNA-gRNA units from several pGRNA vectors with a plant binary vector containing a
conclusionsThis multiplex gRNA expression system enables high-throughput production of a single binary vector and increases the efficiency of plant genome editing.
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