ArticleScientific reports2020
Foreign DNA detection by high-throughput sequencing to regulate genome-edited agricultural products.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Rapid development of naked malting barley germplasm through targeted mutagenesis.Molecular breeding : new strategies in plant improvement · 2025Article
- Off-target sequence variations driven by the intrinsic properties of the Cas-sgRNA-DNA complex in genome editing.PloS one · 2025Article
- Trans-nuclei CRISPR/Cas9: safe approach for genome editing in the edible mushroom excluding foreign DNA sequences.Applied microbiology and biotechnology · 2024Article
- Impact of rice GENERAL REGULATORY FACTOR14h (GF14h) on low-temperature seed germination and its application to breeding.PLoS genetics · 2024Article
- Generation of major glutelin-deficient (GluA, GluB, and GluC) semi-dwarf Koshihikari rice line.Plant cell reports · 2024Article
- Efficient double-flowered gentian plant production using the CRISPR/Cas9 system.Plant biotechnology (Tokyo, Japan) · 2023Article
- Preface to the special issue "Current Status and Future Prospects for the Development of Crop Varieties and Breeding Materials Using Genome Editing Technology".Plant biotechnology (Tokyo, Japan) · 2023Article
- Foreign DNA detection in genome-edited potatoes by high-throughput sequencing.Scientific reports · 2023Article
- Fertilization controls tiller numbers via transcriptional regulation of a MAX1-like gene in rice cultivation.Nature communications · 2023Article
- Regulatory approaches for genome edited agricultural plants in select countries and jurisdictions around the world.Transgenic research · 2021Review
- INSIDER: alignment-free detection of foreign DNA sequences.Computational and structural biotechnology journal · 2021Article
- Real-Time Monitoring of Key Gene Products Involved in Rice Photoperiodic Flowering.Frontiers in plant science · 2021Article
- Simultaneous induction of mutant alleles of two allergenic genes in soybean by using site-directed mutagenesis.BMC plant biology · 2020Article
- Targeted genome editing in tetraploid potato through transient TALEN expression byPlant biotechnology (Tokyo, Japan) · 2020Article
- Preface to the special issue "Technology in tissue culture toward horizon of plant biotechnology".Plant biotechnology (Tokyo, Japan) · 2020Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the advent of several new breeding techniques (NBTs) is revolutionizing agricultural production processes, technical information necessary for their regulation is yet to be provided. Here, we show that high-throughput DNA sequencing is effective for the detection of unintended remaining foreign DNA segments in genome-edited rice. A simple k-mer detection method is presented and validated through a series of computer simulations and real data analyses. The data show that a short foreign DNA segment of 20 nucleotides can be detected and the probability that the segment is overlooked is 10
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