Evidence map›Paper›PMID 32188925›Full record

ArticleScientific reports2020

Cytotoxicity, fluorescence tagging and gene-expression study of CuInS/ZnS QDS - meso (hydroxyphenyl) porphyrin conjugate against human monocytic leukemia cells.

Ncediwe Tsolekile, Sara Nahle, Nkosingiphile Zikalala, Sundararajan Parani, El Hadji Mamour Sakho, Olivier Joubert, Mangaka C Matoetoe, Sandile P Songca, Oluwatobi S Oluwafemi

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Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ncediwe TsolekileDepartment of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein, 2028, Johannesburg, South Africa.
Sara NahleUniversité De Lorraine, Faculté de Pharmacie, F-54001, Nancy Cedex, France.
Nkosingiphile ZikalalaDepartment of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein, 2028, Johannesburg, South Africa.
Sundararajan ParaniDepartment of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein, 2028, Johannesburg, South Africa.
El Hadji Mamour SakhoDepartment of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein, 2028, Johannesburg, South Africa.
Olivier JoubertUniversité De Lorraine, Faculté de Pharmacie, F-54001, Nancy Cedex, France.
Mangaka C MatoetoeDepartment of Chemistry, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 2000, South Africa.
Sandile P SongcaDepartment of Chemistry, University of KwaZulu-Natal, Private Bag X 54001, Durban, 4000, South Africa.
Oluwatobi S OluwafemiDepartment of Chemical Sciences, University of Johannesburg, P. O. Box 17011, Doornfontein, 2028, Johannesburg, South Africa. oluwafemi.oluwatobi@gmail.com.ORCID http://orcid.org/0000-0001-8964-5646

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The toxicity of heavy metals present in binary semiconductor nanoparticles also known as quantum dots (QDs) has hindered their wide applications hence the advent of non-toxic ternary quantum dots. These new group of quantum dots have been shown to possess some therapeutic action against cancer cell lines but not significant enough to be referred to as an ideal therapeutic agent. In this report, we address this problem by conjugating red emitting CuInS/ZnS QDs to a 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin -photosensitizer for improved bioactivities. The glutathione capped CuInS/ZnS QDs were synthesized in an aqueous medium using a kitchen pressure cooker at different Cu: In ratios (1:4 and 1:8) and at varied temperatures (95 °C, 190 °C and 235 °C). Optical properties show that the as-synthesized CuInS/ZnS QDs become red-shifted compared to the core (CuInS) after passivation with emission in the red region while the cytotoxicity study revealed excellent cell viability against normal kidney fibroblasts (BHK21). The highly fluorescent, water-soluble QDs were conjugated to 5,10,15,20-tetrakis(3-hydroxyphenyl)porphyrin (mTHPP) via esterification reactions at room temperature. The resultant water-soluble conjugate was then used for the cytotoxicity, fluorescent imaging and gene expression study against human monocytic leukemia cells (THP-1). Our result showed that the conjugate possessed high cytotoxicity against THP-1 cells with enhanced localized cell uptake compared to the bare QDs. In addition, the gene expression study revealed that the conjugate induced inflammation compared to the QDs as NFKB gene was over-expressed upon cell inflammation while the singlet oxygen (

Indexed as

Metal NanoparticlesPorphyrinsSulfidesZinc CompoundsAntineoplastic AgentsCell Line, TumorHumansLeukemia, MyeloidMicroscopy, ConfocalQuantum DotsSpectrum AnalysisTheranostic NanomedicineAntineoplastic AgentsPorphyrinsSulfidestetra(3-hydroxyphenyl)porphineZinc Compoundszinc sulfide

Identifiers

PMID32188925
PMCPMC7080734

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.