ArticleNature communications2020
Abundance of conserved CRISPR-Cas9 target sites within the highly polymorphic genomes of Anopheles and Aedes mosquitoes.
Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Article
- Genetic divergence in Aedes aegypti mosquitoes potentially associated with enhanced capacity for arbovirus transmission.BMC biology · 2026Article
- Integrating mosquito genomics into simulation modeling: opportunities for better-informed biocontrol.Current opinion in insect science · 2026Review
- Population genomics of Aedes albopictus across remote Pacific islands for genetic biocontrol considerations.PLoS neglected tropical diseases · 2025Article
- CRISPR-Cas9 and Cas12a target site richness reflects genomic diversity in natural populations of Anopheles gambiae and Aedes aegypti mosquitoes.BMC genomics · 2024Article
- Assessing single-locus CRISPR/Cas9-based gene drive variants in the mosquito Aedes aegypti via single-generation crosses and modeling.G3 (Bethesda, Md.) · 2022Article
- Population replacement gene drive characteristics for malaria elimination in a range of seasonal transmission settings: a modelling study.Malaria journal · 2022Article
- Article
- Towards application of CRISPR-Cas12a in the design of modern viral DNA detection tools (Review).Journal of nanobiotechnology · 2022Review
- Gene drives gaining speed.Nature reviews. Genetics · 2022Review
- Population genomics of Drosophila suzukii reveal longitudinal population structure and signals of migrations in and out of the continental United States.G3 (Bethesda, Md.) · 2021Article
- Evaluation of Gene Knockouts by CRISPR as Potential Targets for the Genetic Engineering of the MosquitoThe CRISPR journal · 2021Article
- Population genomics of invasive rodents on islands: Genetic consequences of colonization and prospects for localized synthetic gene drive.Evolutionary applications · 2021Article
- Control of malaria-transmitting mosquitoes using gene drives.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Article
- Monitoring Needs for Gene Drive Mosquito Projects: Lessons From Vector Control Field Trials and Invasive Species.Frontiers in genetics · 2021Article
- Adequacy and sufficiency evaluation of existing EFSA guidelines for the molecular characterisation, environmental risk assessment and post-market environmental monitoring of genetically modified insects containing engineered gene drives.EFSA journal. European Food Safety Authority · 2020Article
- Next-generation gene drive for population modification of the malaria vector mosquito,Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
A number of recent papers report that standing genetic variation in natural populations includes ubiquitous polymorphisms within target sites for Cas9-based gene drive (CGD) and that these "drive resistant alleles" (DRA) preclude the successful application of CGD for managing these populations. Here we report the results of a survey of 1280 genomes of the mosquitoes Anopheles gambiae, An. coluzzii, and Aedes aegypti in which we determine that ~90% of all protein-encoding CGD target genes in natural populations include at least one target site with no DRAs at a frequency of ≥1.0%. We conclude that the abundance of conserved target sites in mosquito genomes and the inherent flexibility in CGD design obviates the concern that DRAs present in the standing genetic variation of mosquito populations will be detrimental to the deployment of this technology for population modification strategies.
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