ArticleClinical and experimental dental research2020
Real-time polymerase chain reaction quantification of the salivary levels of cariogenic bacteria in patients with orthodontic fixed appliances.
Article in Clinical and experimental dental research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.
- White spot lesions, plaque accumulation and salivary caries-associated bacteria in clear aligners compared to fixed orthodontic treatment. A systematic review and meta- analysis.BMC oral health · 2023Pooled it
- Microbial and periodontal shifts induced by orthodontic appliances: a comparative clinical study.Acta odontologica Scandinavica · 2026Article
- Low-Cost Protocol for Quantitative Measurement ofLife (Basel, Switzerland) · 2025Article
- Perception of Dental Caries Risk and Preventive Strategies Among Orthodontic Patients: A Cross-Sectional Survey.Cureus · 2025Article
- Exploring the Correlation between Selected Oral Commensals and Deoxyribonucleic Acid Degradation in Pulp Tissue Extracted from Primary Teeth Stored in Dry and Wet Conditions: AnInternational journal of clinical pediatric dentistry · 2025Article
- Dynamic effects of fixed orthodontic treatment on oral health and oral microbiota: a prospective study.BMC oral health · 2024Article
- Quantitative Determination of Salivary Cariogenic Bacteria in Patients With Fixed Orthodontic Appliances.Cureus · 2024Article
- Oral Prevalence ofPathogens (Basel, Switzerland) · 2024Article
- Microbiome and metabolome associated with white spot lesions in patients treated with clear aligners.Frontiers in cellular and infection microbiology · 2023Article
- Real-time polymerase chain reaction quantification of the salivary levels of cariogenic bacteria in patients with orthodontic fixed appliances.Clinical and experimental dental research · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimThe aim was to investigate the salivary detection frequencies and quantities of caries-associated bacteria from patients with orthodontic brackets.
methodsPatients wearing orthodontic brackets (n = 40, mean age = 26 years) and healthy controls without brackets (n = 40, mean age = 17 years) were enrolled in the study. Saliva samples from each patient was collected. After DNA purification, target species comprising streptococci and a Lactobacillus species were detected and quantified from the samples using polymerase chain reaction (PCR) and real-time quantitative PCR.
resultsDetection frequencies did not differ between the orthodontic patients and the control subjects for any target species except for Streptococcus sobrinus, which showed significantly lower detection rates in orthodontic patients (p < .05). Lactobacillus casei and Streptococcus gordonii were found at the highest detection frequencies with both species being detected in 38 (95%) of the saliva samples of orthodontic patients. Similarly, L. casei and Streptococcus salivarius were the species with highest detection frequencies (35, 87.5%) in the control subjects. Real-time PCR revealed that Streptococcus mutans and S. salivarius quantities were significantly higher in orthodontic patients than in the control subjects (p < .05).
conclusionsApplication of orthodontic brackets for 12 months leads to increased salivary levels of cariogenic bacteria and may serve as a potential risk factor for caries initiation.
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