Evidence map›Paper›PMID 32185887›Full record

ArticleJournal of cellular and molecular medicine2020

Guaiacol suppresses osteoclastogenesis by blocking interactions of RANK with TRAF6 and C-Src and inhibiting NF-κB, MAPK and AKT pathways.

Xin Zhi, Chao Fang, Yanqiu Gu, Huiwen Chen, Xiaofei Chen, Jin Cui, Yan Hu, Weizong Weng, Qirong Zhou, Yajun Wang and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
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  10. Frontiers in pharmacology · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 1 institution in 2 countries.

Xin ZhiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Chao FangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Yanqiu GuDepartment of Pharmacy, Shanghai 9th People's Hospital, Shanghai, China.
Huiwen ChenDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Xiaofei ChenSchool of Pharmacy, Naval Military Medical University, Shanghai, China.
Jin CuiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Yan HuDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Weizong WengDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Qirong ZhouDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Yajun WangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Yao WangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Hao JiangDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Xiaoqun LiDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Liehu CaoDepartment of Orthopedics Trauma, Shanghai Luodian Hospital, Shanghai, China.
Xiao ChenDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.
Jiacan SuDepartment of Orthopedics Trauma, Shanghai Changhai Hospital, Naval Military Medical University, Shanghai, China.ORCID 0000-0001-7080-263X
Second Military Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Angelica sinensis (AS; Dang Gui), a traditional Chinese herb, has for centuries been used for the treatment of bone diseases, including osteoporosis and osteonecrosis. However, the effective ingredient and underlying mechanisms remain elusive. Here, we identified guaiacol as the active component of AS by two-dimensional cell membrane chromatography/C18 column/time-of-flight mass spectrometry (2D CMC/C18 column/TOFMS). Guaiacol suppressed osteoclastogenesis and osteoclast function in bone marrow monocytes (BMMCs) and RAW264.7 cells in vitro in a dose-dependent manner. Co-immunoprecipitation indicated that guaiacol blocked RANK-TRAF6 association and RANK-C-Src association. Moreover, guaiacol prevented phosphorylation of p65, p50, IκB (NF-κB pathway), ERK, JNK, c-fos, p38 (MAPK pathway) and Akt (AKT pathway), and reduced the expression levels of Cathepsin K, CTR, MMP-9 and TRAP. Guaiacol also suppressed the expression of nuclear factor of activated T-cells cytoplasmic 1(NFATc1) and the RANKL-induced Ca

Indexed as

AdipogenesisAnimalsBone Marrow CellsBone ResorptionCell ProliferationCSK Tyrosine-Protein KinaseFemaleGuaiacolMAP Kinase Signaling SystemMiceMice, Inbred C57BLNF-kappa BNF-kappa B p50 SubunitOsteoclastsOsteogenesisProto-Oncogene Proteins c-aktAkt1 protein, mouseCSK Tyrosine-Protein KinaseGuaiacolNF-kappa BNF-kappa B p50 SubunitNfkb1 protein, mouseProto-Oncogene Proteins c-aktReceptor Activator of Nuclear Factor-kappa BTNF Receptor-Associated Factor 6Tnfrsf11a protein, mouseTRAF6 protein, mouseguaiacolosteoclastogenesisosteoporosisrankl

Identifiers

PMID32185887
PMCPMC7205840
OpenAlexW3011096695

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.