Evidence map›Paper›PMID 32185871›Full record

ArticleAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons2020

Discovery of non-HLA antibodies associated with cardiac allograft rejection and development and validation of a non-HLA antigen multiplex panel: From bench to bedside.

Carrie L Butler, Michelle J Hickey, Ning Jiang, Ying Zheng, David Gjertson, Qiuheng Zhang, Ping Rao, Gregory A Fishbein, Martin Cadeiras, Mario C Deng and 19 more

Open access · greenAbstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 51 citations in OpenAlex.

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  9. Surveillance donor-specific antibody and pathologic antibody-mediated rejection testing in heart transplant patients in the contemporary era.The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation · 2025
    Article
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  19. The evolving use of biomarkers in heart transplantation: Consensus of an expert panel.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2023
    Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

29 authors at 9 institutions in 1 country.

Carrie L ButlerDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.ORCID 0000-0001-7783-7819
Michelle J HickeyDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
Ning JiangImmucor, Inc., Norcross, Georgia, USA.
Ying ZhengDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
David GjertsonDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
Qiuheng ZhangDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
Ping RaoDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.
Gregory A FishbeinDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.ORCID 0000-0002-3632-5654
Martin CadeirasDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Mario C DengDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Hector L BanchsCardiovascular Center of Puerto Rico and the Caribbean Transplant Program, Carolina, Puerto Rico, USA.
Guillermo TorreHouston Methodist Hospital Research Institution, Houston, Texas, USA.
David DeNofrioTufts Medical Center, Boston, Massachusetts, USA.
Howard J EisenDrexel University College of Medicine, Philadelphia, Pennsylvania, USA.ORCID 0000-0003-1149-4344
Jon KobashigawaCedars-Sinai Heart Institute, Los Angeles, California, USA.
Randall C StarlingDepartment of Cardiovascular Medicine, Cleveland Clinic, Cleveland, Ohio, USA.
Abdallah KfouryIntermountain Medical Center, Salt Lake City, Utah, USA.
Adrian Van BakelDivision of Cardiology, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina, USA.
Gregory EwaldDepartment of Medicine, Washington University, St. Louis, Missouri, USA.
Ivan BalazsImmucor, Inc., Norcross, Georgia, USA.
Arnold S BaasDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Daniel CruzDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Reza ArdehaliDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Reshma BiniwaleDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Murray KwonDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Abbas ArdehaliDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Ali NsairDepartment of Medicine, Division of Cardiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA.
Bryan RayImmucor, Inc., Norcross, Georgia, USA.
Elaine F ReedDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA.ORCID 0000-0002-3524-545X
University of California, Los Angeles · USImmucor (United States) · USCleveland Clinic · USDrexel University · USIntermountain Medical Center · USMedical University of South Carolina · USMethodist Hospital · USTufts Medical Center · USWashington University in St. Louis · US

Funding

The role of HLA and its coreceptors in endothelial cell activation and leukocyte recruitment in antibody-mediated transplant rejectionR01AI135201 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Robert L Fairchild, ELAINE F REED · 2018 to 2026
$5.3M
Anti-MHC Effects on EndotheliumR01AI042819 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI REED, ELAINE F · 1999 to 2014
$4.0M
Translational Research Training in Pediatric NephrologyT32DK104687 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SALUSKY, ISIDRO B. · 2015 to 2019
$879k
NIAID NIH HHS R01 AI042819NIAID NIH HHS R01 AI135201NIDDK NIH HHS T32 DK104687-02
6 · The paper itself

Abstract

We analyzed humoral immune responses to nonhuman leukocyte antigen (HLA) after cardiac transplantation to identify antibodies associated with allograft rejection. Protein microarray identified 366 non-HLA antibodies (>1.5 fold, P < .5) from a discovery cohort of HLA antibody-negative, endothelial cell crossmatch-positive sera obtained from 12 cardiac allograft recipients at the time of biopsy-proven rejection. From these, 19 plasma membrane proteins and 10 autoantigens identified from gene ontology analysis were combined with 48 proteins identified through literature search to generate a multiplex bead array. Longitudinal sera from a multicenter cohort of adult cardiac allograft recipients (samples: n = 477 no rejection; n = 69 rejection) identified 18 non-HLA antibodies associated with rejection (P < .1) including 4 newly identified non-HLA antigenic targets (DEXI, EMCN, LPHN1, and SSB). CART analysis showed 5/18 non-HLA antibodies distinguished rejection vs nonrejection. Antibodies to 4/18 non-HLA antigens synergize with HLA donor-specific antibodies and significantly increase the odds of rejection (P < .1). The non-HLA panel was validated using an independent adult cardiac transplant cohort (n = 21 no rejection; n = 42 rejection, >1R) with an area under the curve of 0.87 (P < .05) with 92.86% sensitivity and 66.67% specificity. We conclude that multiplex bead array assessment of non-HLA antibodies identifies cardiac transplant recipients at risk of rejection.

Indexed as

Graft RejectionHeart TransplantationAllograftsAntibodiesHLA AntigensAntibodiesHLA Antigensautoantibodyautoantigenclinical research/practiceheart transplantation/cardiologyhistocompatibilityimmunogeneticsmicroarray/protein arrayorgan transplantation in generalrejectiontranslational research/science

Identifiers

PMID32185871
PMCPMC7494540
OpenAlexW3012295850

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.