Evidence map›Paper›PMID 32184938›Full record

ArticleWorld journal of stem cells2020

Comparison between the therapeutic effects of differentiated and undifferentiated Wharton's jelly mesenchymal stem cells in rats with streptozotocin-induced diabetes.

Chen-Yuan Hsiao, Tien-Hua Chen, Ben-Shian Huang, Po-Han Chen, Cheng-Hsi Su, Jia-Fwu Shyu, Pei-Jiun Tsai

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of stem cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.3field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Chen-Yuan HsiaoGraduate Institute of Medical Sciences, National Defense Medical Center, Taipei 114, Taiwan.
Tien-Hua ChenInstitute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, Taipei 112, Taiwan.
Ben-Shian HuangDepartment of Obstetrics and Gynecology, Veterans General Hospital, Taipei 112, Taiwan.
Po-Han ChenInstitute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, Taipei 112, Taiwan.
Cheng-Hsi SuDepartment of Surgery, Cheng Hsin General Hospital, Taipei 112, Taiwan.
Jia-Fwu ShyuDepartment of Biology and Anatomy, National Defense Medical Center, Taipei 114, Taiwan.
Pei-Jiun TsaiInstitute of Anatomy and Cell Biology, School of Medicine, National Yang Ming University, Taipei 112, Taiwan.
National Defense Medical Center · TWNational Yang Ming Chiao Tung University · TWCheng Hsin General Hospital · TWNational Yang Ming Chiao Tung University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDespite the availability of current therapies, including oral antidiabetic drugs and insulin, for controlling the symptoms caused by high blood glucose, it is difficult to cure diabetes mellitus, especially type 1 diabetes mellitus.

aimCell therapies using mesenchymal stem cells (MSCs) may be a promising option. However, the therapeutic mechanisms by which MSCs exert their effects, such as whether they can differentiate into insulin-producing cells (IPCs) before transplantation, are uncertain.

methodsIn this study, we used three types of differentiation media over 10 d to generate IPCs from human Wharton's jelly MSCs (hWJ-MSCs). We further transplanted the undifferentiated hWJ-MSCs and differentiated IPCs derived from them into the portal vein of rats with streptozotocin-induced diabetes, and recorded the physiological and pathological changes.

resultsUsing fluorescent staining and C-peptide enzyme-linked immunoassay, we were able to successfully induce the differentiation of hWJ-MSCs into IPCs. Transplantation of both IPCs derived from hWJ-MSCs and undifferentiated hWJ-MSCs had the therapeutic effect of ameliorating blood glucose levels and improving intraperitoneal glucose tolerance tests. The transplanted IPCs homed to the pancreas and functionally survived for at least 8 wk after transplantation, whereas the undifferentiated hWJ-MSCs were able to improve the insulitis and ameliorate the serum inflammatory cytokine in streptozotocin-induced diabetic rats.

conclusionDifferentiated IPCs can significantly improve blood glucose levels in diabetic rats due to the continuous secretion of insulin by transplanted cells that survive in the islets of diabetic rats. Transplantation of undifferentiated hWJ-MSCs can significantly improve insulitis and re-balance the inflammatory condition in diabetic rats with only a slight improvement in blood glucose levels.

Indexed as

Anti-inflammatoryDiabetes mellitusDifferentiationHuman Wharton’s jelly mesenchymal stem cellInsulin-producing cellsRegeneration therapy

Identifiers

PMID32184938
PMCPMC7062039
OpenAlexW3008837089

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.