ArticleFrontiers in microbiology2020
Regulation of Microbial Metabolic Rates Using CRISPR Interference With Expanded PAM Sequences.
Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 14 citations in OpenAlex.
- AlPaCas: allele-specific CRISPR gene editing through a protospacer-adjacent-motif (PAM) approach.Nucleic acids research · 2024Article
- Use of paired Cas9-NG nickase and truncated sgRNAs for single-nucleotide microbial genome editing.Frontiers in genome editing · 2024Article
- New Target Gene Screening Using Shortened and Random sgRNA Libraries in Microbial CRISPR Interference.ACS synthetic biology · 2023Article
- Miniature CRISPR-Cas12f1-Mediated Single-Nucleotide Microbial Genome Editing Using 3'-Truncated sgRNA.The CRISPR journal · 2023Article
- Integrase-mediated differentiation circuits improve evolutionary stability of burdensome and toxic functions in E. coli.Nature communications · 2022Article
- Advances in Accurate Microbial Genome-Editing CRISPR Technologies.Journal of microbiology and biotechnology · 2021Review
- Mismatch Intolerance of 5'-Truncated sgRNAs in CRISPR/Cas9 Enables Efficient Microbial Single-Base Genome Editing.International journal of molecular sciences · 2021Article
- dCas9 regulator to neutralize competition in CRISPRi circuits.Nature communications · 2021Article
- Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference.Journal of microbiology and biotechnology · 2020Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome-editing CRISPR/Cas9 technology has led to the development of artificial transcriptional repressors, also known as CRISPR interference (CRISPRi). The deactivated Cas9 (dCas9) protein guided by crRNA can specifically bind to target DNA sequences, including promoters and operators, without DNA cleavage. Protospacer adjacent motif (PAM) sequence dependence may be disadvantageous in the design of target-specific CRISPRi, as the PAM sequence is essential for DNA cleavage by the CRISPR/Cas9 system. We constructed a chromosomally integrated dCas9 system (Δ
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Registered trials
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