ArticleProceedings of the National Academy of Sciences of the United States of America2020
Specialized cytonemes induce self-organization of stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 45 citations in OpenAlex.
- Regulation and function of specialized membrane protrusions in intercellular communication.Nature reviews. Molecular cell biology · 2026Review
- Ovarian germline stem cell dedifferentiation is cytoneme dependent.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Filopodia are essential for steroid release.Nature communications · 2025Article
- Making cells inter-connected for signaling communication: a developmental view of cytonemes.Cell communication and signaling : CCS · 2025Review
- WNT7A-positive dendritic cytonemes control synaptogenesis in cortical neurons.Development (Cambridge, England) · 2024Article
- The actin-bundling protein Fascin-1 modulates ciliary signalling.Journal of molecular cell biology · 2023Article
- Human embryo live imaging reveals nuclear DNA shedding during blastocyst expansion and biopsy.Cell · 2023Article
- Pericyte-Glioblastoma Cell Interaction: A Key Target to Prevent Glioblastoma Progression.Cells · 2023Review
- Non-Classical Intercellular Communications: Basic Mechanisms and Roles in Biology and Medicine.International journal of molecular sciences · 2023Review
- Adult tissue-specific stem cell interaction: novel technologies and research advances.Frontiers in cell and developmental biology · 2023Review
- Direct Cell-Cell Communication via Membrane Pores, Gap Junction Channels, and Tunneling Nanotubes: Medical Relevance of Mitochondrial Exchange.International journal of molecular sciences · 2022Review
- Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche.Nature communications · 2022Article
- Regulatory mechanisms of cytoneme-based morphogen transport.Cellular and molecular life sciences : CMLS · 2022Review
- Joint single-cell multiomic analysis in Wnt3a induced asymmetric stem cell division.Nature communications · 2021Article
- Spindle positioning and its impact on vertebrate tissue architecture and cell fate.Nature reviews. Molecular cell biology · 2021Review
- Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach.STAR protocols · 2021Article
- Regulated delivery controlseLife · 2021Article
- Review
- Wnt- and glutamate-receptors orchestrate stem cell dynamics and asymmetric cell division.eLife · 2021Article
- Cytonemes with complex geometries and composition extend into invaginations of target cells.The Journal of cell biology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Spatial cellular organization is fundamental for embryogenesis. Remarkably, coculturing embryonic stem cells (ESCs) and trophoblast stem cells (TSCs) recapitulates this process, forming embryo-like structures. However, mechanisms driving ESC-TSC interaction remain elusive. We describe specialized ESC-generated cytonemes that react to TSC-secreted Wnts. Cytoneme formation and length are controlled by actin, intracellular calcium stores, and components of the Wnt pathway. ESC cytonemes select self-renewal-promoting Wnts via crosstalk between Wnt receptors, activation of ionotropic glutamate receptors (iGluRs), and localized calcium transients. This crosstalk orchestrates Wnt signaling, ESC polarization, ESC-TSC pairing, and consequently synthetic embryogenesis. Our results uncover ESC-TSC contact-mediated signaling, reminiscent of the glutamatergic neuronal synapse, inducing spatial self-organization and embryonic cell specification.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.