A topological analysis of difference topology experiments of condensin with topoisomerase II.

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Article in Biology open, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 0 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Soojeong KimYonsei University, University College, Incheon 21983, South Korea.ORCID 0000-0003-0543-6877

Isabel K DarcyDepartment of Mathematics, University of Iowa, Iowa City 52242, USA isabel-darcy@uiowa.edu.ORCID 0000-0001-5068-1252

University of Iowa · USYonsei University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An experimental technique called difference topology combined with the mathematics of tangle analysis has been used to unveil the structure of DNA bound by the Mu transpososome. However, difference topology experiments can be difficult and time consuming. We discuss a modification that greatly simplifies this experimental technique. This simple experiment involves using a topoisomerase to trap DNA crossings bound by a protein complex and then running a gel to determine the crossing number of the knotted product(s). We develop the mathematics needed to analyze the results and apply these results to model the topology of DNA bound by 13S condensin and by the condensin MukB.

Indexed as

Models, TheoreticalAdenosine TriphosphatasesDNADNA-Binding ProteinsDNA Topoisomerases, Type IIMacromolecular SubstancesMultiprotein ComplexesNucleic Acid ConformationStructure-Activity RelationshipAdenosine Triphosphatasescondensin complexesDNADNA-Binding ProteinsDNA Topoisomerases, Type IIMacromolecular SubstancesMultiprotein ComplexesCondensinDifference topology experimentDNA topologyTangle analysis

Identifiers

PMID32184229

DOI10.1242/bio.048603

PMCPMC7132813

OpenAlexW3012128499

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.