Evidence map›Paper›PMID 32183976›Full record

ArticleMethods in cell biology2020

Method for live-cell super-resolution imaging of mitochondrial cristae and quantification of submitochondrial membrane potentials.

Dane M Wolf, Mayuko Segawa, Orian S Shirihai, Marc Liesa

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  5. Mitochondrial FEMBO reports · 2021
    Article
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Dane M WolfDepartment of Medicine, Endocrinology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States; Graduate Program in Nutrition and Metabolism, Graduate Medical Sciences, Boston University School of Medicine, Boston, MA, United States.
Mayuko SegawaDepartment of Medicine, Endocrinology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States.
Orian S ShirihaiDepartment of Medicine, Endocrinology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States. Electronic address: oshirihai@mednet.ucla.edu.
Marc LiesaDepartment of Medicine, Endocrinology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States; Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States; Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, United States. Electronic address: mliesa@mednet.ucla.edu.
University of California, Los Angeles · US

Funding

Transgenic & Knock-out MouseP30DK063491 · NIDDK · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI PETER J TONTONOZ · 2003 to 2026
$40.4M
Mitochondrial regulation of energy efficiencyR01DK099618 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CORKEY, BARBARA E., SHIRIHAI, ORIAN S · 2014 to 2018
$1.8M
Role of the heme-related mitochondrial antioxidant ABCB10 in alcoholic liver diseaseR01AA026914 · NIAAA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SHIRIHAI, ORIAN S · 2019 to 2022
$1.4M
NIAAA NIH HHS R01 AA026914NIDDK NIH HHS P30 DK063491NIDDK NIH HHS R01 DK099618
6 · The paper itself

Abstract

The emergence of diffraction-unlimited live-cell imaging technologies has enabled the examination of mitochondrial form and function in unprecedented detail. We recently developed an approach for visualizing the inner mitochondrial membrane and determined that cristae membranes possess distinct mitochondrial membrane potentials, representing unique bioenergetic subdomains within the same organelle. Here, we outline a methodology for resolving cristae and inner boundary membranes using the LSM880 with Airyscan. Furthermore, we demonstrate how to analyze TMRE fluorescence intensity using the Nernst equation to calculate membrane potentials of individual cristae. Altogether, using these new techniques to study the electrochemical properties of the cristae can help to gain deeper insight into the still elusive nature of the mitochondrion.

Indexed as

Membrane Potential, MitochondrialCell SurvivalHeLa CellsHumansImaging, Three-DimensionalMitochondriaMitochondrial MembranesAiryscanCristaeLive-cell imagingMitochondrial membrane potentialSuper-resolution imaging

Identifiers

PMID32183976
PMCPMC7216778
OpenAlexW3009738747

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.