Evidence map›Paper›PMID 32183974›Full record

ArticleMethods in cell biology2020

In vitro and in vivo assays for mitochondrial fission and fusion.

Suzanne Hoppins, Laura L Lackner, Jason E Lee, Jason A Mears

Abstract read
In one paragraph

Article in Methods in cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Insights fromFrontiers in neuroscience · 2023
    Review
  5. Review
  6. NMR identification of a conserved Drp1 cardiolipin-binding motif essential for stress-induced mitochondrial fission.Proceedings of the National Academy of Sciences of the United States of America · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Suzanne HoppinsDepartment of Biochemistry, University of Washington School of Medicine, Seattle, WA, United States. Electronic address: shoppins@uw.edu.
Laura L LacknerDepartment of Molecular Biosciences, Northwestern University, Evanston, IL, United States. Electronic address: laura.lackner@northwestern.edu.
Jason E LeeDepartment of Molecular, Cellular and Developmental Biology, University of Colorado at Boulder, Boulder, CO, United States. Electronic address: jason.lee@colorado.edu.
Jason A MearsDepartment of Pharmacology, Center for Mitochondrial Diseases, and Cleveland Center for Membrane and Structural Biology, Case Western Reserve University School of Medicine, Cleveland, OH, United States. Electronic address: jason.mears@case.edu.

Funding

Determining the mechanism of mitochondrial outer membrane fusionR01GM118509 · NIGMS · UNIVERSITY OF WASHINGTON · PI HOPPINS, SUZANNE C · 2017 to 2025
$3.3M
Investigating the molecular mechanism of mitochondrial tetheringR01GM120303 · NIGMS · NORTHWESTERN UNIVERSITY · PI LACKNER, LAURA L · 2017 to 2025
$2.9M
Supplement: Defining Molecular Interactions that Drive Mitochondrial FissionR01GM125844 · NIGMS · CASE WESTERN RESERVE UNIVERSITY · PI Jason Mears · 2018 to 2026
$2.8M
Mitochondrial Dynamics in Brain TumorInitiating CellsR01CA208516 · NCI · CASE WESTERN RESERVE UNIVERSITY · PI MEARS, JASON · 2017 to 2021
$1.6M
NCI NIH HHS R01 CA208516NIGMS NIH HHS R01 GM118509NIGMS NIH HHS R01 GM120303NIGMS NIH HHS R01 GM125844
6 · The paper itself

Abstract

Mitochondria are required for cell survival and are best known for their role in energy production. These organelles also participate in many other biological processes that are critical for cellular function, and thus, play a central role in cellular life and death decisions. In a majority of cell types, mitochondria form highly dynamic, reticular networks. Maintaining the shape of these complex, ever-changing networks is critical for mitochondrial and cellular function, and requires the conserved activities of mitochondrial fission and fusion. Great advances in our knowledge about the molecular machines that mediate these dynamic activities have been made over the past 2 decades. These advances have been driven by the use of highly complementary in vitro and in vivo approaches that have proven extremely powerful for studying the complex membrane remodeling processes that drive fission and fusion of the organelle. In this chapter, we detail current methods used to examine the mechanisms and regulation of mitochondrial fission and fusion in vitro and in vivo.

Indexed as

Mitochondrial DynamicsAnimalsBiological AssayChromatography, AffinityDynaminsFluorescenceGuanosine TriphosphateHeLa CellsHumansLiposomesMiceMitochondriaPhotobleachingDnm1l protein, mouseDynaminsGuanosine TriphosphateLiposomesMitochondrial divisionMitochondrial dynamicsMitochondrial fissionMitochondrial fusion

Identifiers

PMID32183974
PMCPMC7863990

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.