ArticleParasitology2020
Free-living amoebae and their relationship to air quality in hospital environments: characterization of
Article in Parasitology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A history of over 40 years of potentially pathogenic free-living amoeba studies in Brazil - a systematic review.Memorias do Instituto Oswaldo Cruz · 2022Pooled it
- Contamination of medical devices and hospital environments with free-living amoebae: Evidence from hospitals in Northwestern Iran.PloS one · 2026Article
- Prevalence of free-living amoebae in five rivers associated with high human activity in Tehran province, Iran.Journal of water and health · 2025Article
- Review
- Comparative genomic analysis ofmSphere · 2024Article
- Epidemiology of and Genetic Factors Associated withPathogens (Basel, Switzerland) · 2024Review
- Diagnosis ofDiagnostics (Basel, Switzerland) · 2023Review
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Recognition of Cell Wall Mannosylated Components as a Conserved Feature for Fungal Entrance, Adaptation and Survival Within Trophozoites ofFrontiers in cellular and infection microbiology · 2022Article
- Isolation and molecular identification ofParasite epidemiology and control · 2021Article
- Molecular Identification of Pathogenic Free-Living Amoeba from Household Biofilm Samples in Iran: A Risk Factor forMicroorganisms · 2021Article
- Growth comparison ofHeliyon · 2020Article
- Acanthamoeba Infection in a Hematopoietic Cell Transplant Recipient: Challenges in Diagnosis, Management, and Source Identification.Transplant infectious disease : an official journal of the Transplantation SocietyArticle
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9 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Free-living amoebae (FLA) are widely dispersed in the environment, can cause opportunistic and non-opportunistic infections in humans and other animals. The aim of the present study was characterize FLA obtained from air-conditioners of a public hospital in the city of Florianópolis, SC, Brazil. Fifty-four dust samples were collected of air conditioners, and were inoculated on 1.5% non-nutrient agar, overlaid with layers of Escherichia coli. Subsequently the isolates were axenised in PYG growth medium. The morphological and molecular characterization of the isolates was performed, as well as the tolerance (physiological) assays were used to evaluate the pathogenic potential. The results revealed the presence of FLA in 42 (77.8%) of the collected samples. Of these, 39 (92.9%) axenic isolates of FLA were obtained for morphological and genotypic studies. All the isolates characterized belong to the genus Acanthamoeba. Nineteen (48.7%) isolates belong to the genotype T4, 16 (41.0%) to the T5 genotype and 4 (10.3%) to genotype T11. Seven (18.0%) isolates were considered potentially pathogenic in tolerance assays. These findings require attention, considering the isolation environment and immunocompromised characteristics of many hospitalized patients.
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