ArticleBMC ophthalmology2020
Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach.
Article in BMC ophthalmology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Leucine-rich α-2-glycoprotein 1 initiates the onset of diabetic retinopathy in mice.Science translational medicine · 2025Article
- Kinetic changes in microglia-related retinal transcripts in experimental autoimmune uveoretinitis (EAU) of B10.RIII mice.Journal of neuroinflammation · 2025Article
- Neutrophil extracellular traps potentiate effector T cells via endothelial senescence in uveitis.JCI insight · 2025Article
- Oncostatin M Reduces Pathological Neovascularization in the Retina Through Müller Cell Activation.Investigative ophthalmology & visual science · 2024Article
- The disruptive role of LRG1 on the vasculature and perivascular microenvironment.Frontiers in cardiovascular medicine · 2024Review
- Crystallin β-b2 promotes retinal ganglion cell protection in experimental autoimmune uveoretinitis.Frontiers in cellular neuroscience · 2024Article
- High salt diet alleviates disease severity in native experimental autoimmune uveitis.Frontiers in ophthalmology · 2024Article
- Correction: Retinal endothelial cell phenotypic modifications during experimental autoimmune uveitis: a transcriptomic approach.BMC ophthalmology · 2023Article
- LRG1 as a novel therapeutic target in eye disease.Eye (London, England) · 2022Review
- Integrated Transcriptome Analysis of Iris Tissues in Experimental Autoimmune Uveitis.Frontiers in genetics · 2022Article
- Distinct Mechanisms of Human Retinal Endothelial Barrier Modulation In Vitro by Mediators of Diabetes and Uveitis.Life (Basel, Switzerland) · 2021Article
- Primed Mycobacterial Uveitis (PMU) as a Model for Post-Infectious Uveitis.Journal of visualized experiments : JoVE · 2021Article
- Inflammatory resolution and vascular barrier restoration after retinal ischemia reperfusion injury.Journal of neuroinflammation · 2021Article
- AMP-activated protein kinase is a key regulator of acute neurovascular permeability.Journal of cell science · 2021Article
- Review
- Prednisone Reprograms the Transcriptional Immune Cell Landscape in CNS Autoimmune Disease.Frontiers in immunology · 2021Article
- Galectins in the Pathogenesis of Common Retinal Disease.Frontiers in pharmacology · 2021Review
- Research Progress on Leucine-Rich Alpha-2 Glycoprotein 1: A Review.Frontiers in pharmacology · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundBlood-retinal barrier cells are known to exhibit a massive phenotypic change during experimental autoimmune uveitis (EAU) development. In an attempt to investigate the mechanisms of blood-retinal barrier (BRB) breakdown at a global level, we studied the gene regulation of total retinal cells and retinal endothelial cells during non-infectious uveitis.
methodsRetinal endothelial cells were isolated by flow cytometry either in Tie2-GFP mice (CD31
resultsRetinal endothelial cell sorting in wild type C57BL/6 mice was validated by comparative transcriptome analysis with retinal endothelial cells sorted from Tie2-GFP mice, which express GFP under the control of the endothelial-specific receptor tyrosine kinase promoter Tie2. RNA-Seq analysis of total retinal cells mainly brought to light upregulation of genes involved in antigen presentation and T cell activation during EAU. Specific transcriptome analysis of retinal endothelial cells allowed us to identify 82 genes modulated in retinal endothelial cells during EAU development. Protein expression of 5 of those genes (serpina3n, lcn2, ackr1, lrg1 and lamc3) was validated at the level of inner BRB cells.
conclusionThose data not only confirm the involvement of known pathogenic molecules but further provide a list of new candidate genes and pathways possibly implicated in inner BRB breakdown during non-infectious posterior uveitis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.