ArticlePathogens (Basel, Switzerland)2020
Viromes of Ten Alfalfa Plants in Australia Reveal Diverse Known Viruses and a Novel RNA Virus.
Article in Pathogens (Basel, Switzerland), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- An Innovative Binding-Protein-Based dsRNA Extraction Method: Comparison of Cost-Effectiveness of Virus Detection Methods Using High-Throughput Sequencing.Molecular ecology resources · 2025Article
- NanoViromics: long-read sequencing of dsRNA for plant virus and viroid rapid detection.Frontiers in microbiology · 2023Article
- In-silico prediction of RT-qPCR-high resolution melting for broad detection of emaraviruses.PloS one · 2023Article
- Article
- A Novel Flavi-like Virus in Alfalfa (Viruses · 2022Article
- An Annotated List of Legume-Infecting Viruses in the Light of Metagenomics.Plants (Basel, Switzerland) · 2021Review
- The Bunyavirales: The Plant-Infecting Counterparts.Viruses · 2021Review
- Occurrence, Distribution, and Genetic Diversity of Alfalfa (Frontiers in microbiology · 2021Article
- The Plant Negative-Sense RNA Virosphere: Virus Discovery Through New Eyes.Frontiers in microbiology · 2020Review
- High-Throughput Sequencing for Deciphering the Virome of Alfalfa (Frontiers in microbiology · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Alfalfa plants in the field can display a range of virus-like symptoms, especially when grown over many years for seed production. Most known alfalfa viruses have RNA genomes, some of which can be detected using diagnostic assays, but many viruses of alfalfa are not well characterized. This study aims to identify the RNA and DNA virus complexes associated with alfalfa plants in Australia. To maximize the detection of RNA viruses, we purified double-stranded RNA (dsRNA) for high throughput sequencing and characterized the viromes of ten alfalfa samples that showed diverse virus-like symptoms. Using Illumina sequencing of tagged cDNA libraries from immune-captured dsRNA, we identified sequences of the single-stranded RNA viruses, alfalfa mosaic virus (AMV), bean leafroll virus, a new emaravirus tentatively named alfalfa ringspot-associated virus, and persistent dsRNA viruses belonging to the families
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Registered trials
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