ArticleChembiochem : a European journal of chemical biology2020
Surface Display of Complex Enzymes by in Situ SpyCatcher-SpyTag Interaction.
Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Engineering of Surface Display Systems inBiotech (Basel (Switzerland)) · 2026Article
- Engineered Bacterial Membranes as Next-Generation Platforms for Cancer Immunotherapy.Small science · 2026Review
- From tumor targeting to tumor accessibility: surface-engineered bacteria as precision living therapeutics for translational medicine.Journal of hematology & oncology · 2026Review
- Orthogonal Tri-Modular Coiled-Coil Assembly for Programmable Multi-Cargo Display on Escherichia coli Nissle 1917.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Yeast Molecular Display for the Screening and Production of Immunological Proteins Using Nanobody as an Example.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Outer membrane vesicles as a versatile nanoplatform for advanced vaccine development and immunotherapy.Frontiers in immunology · 2026Review
- A carbonic anhydrase-based nanogel for cyanobacterial growth enhancement.Materials today. Bio · 2025Article
- Surface Display Technologies for Whole-Cell Biocatalysts: Advances in Optimization Strategies, Food Applications, and Future Perspectives.Foods (Basel, Switzerland) · 2025Review
- Extracellular Bacterial Production of DNA Hydrogels-Toward Engineered Living Materials.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Surface display of eugenol oxidase and dioxygenase complex as a sustainable biocatalyst for efficient bioconversion of lignin-derived 4-n-propylguaiacol to vanillin.Microbial cell factories · 2025Article
- Living Bacteria: A New Vehicle for Vaccine Delivery in Cancer Immunotherapy.International journal of molecular sciences · 2025Review
- Biodegradation of combined pollutants of polyethylene terephthalate and phthalate esters by esterase-integratedSynthetic and systems biotechnology · 2025Article
- Genetically surface-modifiedBiotechnology reports (Amsterdam, Netherlands) · 2024Article
- Display of PETase on the Cell Surface of Escherichia coli Using the Anchor Protein PgsA.Applied biochemistry and biotechnology · 2024Article
- Overcoming Protein Orientation Mismatch Enables Efficient Nanoscale Light-Driven ATP Production.ACS synthetic biology · 2024Article
- Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display.Scientific reports · 2023Article
- Bacterial biofilm functionalization through Bap amyloid engineering.NPJ biofilms and microbiomes · 2022Article
- A Modular System for the Rapid Comparison of Different Membrane Anchors for Surface Display on Escherichia coli.Chembiochem : a European journal of chemical biology · 2022Article
- Development of a yeast cell surface display method using the SpyTag/SpyCatcher system.Scientific reports · 2021Article
- Surface Display of Complex Enzymes by in Situ SpyCatcher-SpyTag Interaction.Chembiochem : a European journal of chemical biology · 2020Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The display of complex proteins on the surface of cells is of great importance for protein engineering and other fields of biotechnology. Herein, we describe a modular approach, in which the membrane anchor protein Lpp-OmpA and a protein of interest (passenger) are expressed independently as genetically fused SpyCatcher and SpyTag units and assembled in situ by post-translational coupling. Using fluorescent proteins, we first demonstrate that this strategy allows the construct to be installed on the surface of E. coli cells. The scope of our approach was then demonstrated by using three different functional enzymes, the stereoselective ketoreductase Gre2p, the homotetrameric glucose 1-dehydrogenase GDH, and the bulky heme- and diflavin-containing cytochrome P450 BM3 (BM3). In all cases, the SpyCatcher-SpyTag method enabled the generation of functional whole-cell biocatalysts, even for the bulky BM3, which could not be displayed by conventional fusion with Lpp-OmpA. Furthermore, by using a GDH variant carrying an internal SpyTag, the system could be used to display an enzyme with unmodified N- and C-termini.
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