ArticlePloS one2020
Systematic re-evaluation of the long-used standard protocol of urease-dependent metabolome sample preparation.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Integrated Microbiome and Metabolomic Profiling to Identify Potential Biomarkers of Major Depressive Disorder.Journal of microbiology and biotechnology · 2026Article
- Integrated Metabolomics and Microbial Profiling in Patients with Irritable Bowel Syndrome.Journal of microbiology and biotechnology · 2026Article
- Prediction of irritable bowel syndrome by integrating urine metabolites and gut microbiota.Scientific reports · 2025Article
- A GC-MS-Based Urinary Metabolomic Profiling to Identify Potential Biomarkers of Metabolic Syndrome.ACS omega · 2025Article
- A Taguchi Design of Experiments Approach for Untargeted Metabolomics Sample Preparation Optimization.Journal of biomolecular techniques : JBT · 2025Article
- Integration of metabolomics and other omics: from microbes to microbiome.Applied microbiology and biotechnology · 2024Review
- Discovery of urinary biosignatures for tuberculosis and nontuberculous mycobacteria classification using metabolomics and machine learning.Scientific reports · 2024Article
- Optimising a urinary extraction method for non-targeted GC-MS metabolomics.Scientific reports · 2023Article
- Metabolomic profiling to identify early urinary biomarkers and metabolic pathway alterations in autosomal dominant polycystic kidney disease.American journal of physiology. Renal physiology · 2023Article
- Article
- A review of strategies for untargeted urinary metabolomic analysis using gas chromatography-mass spectrometry.Metabolomics : Official journal of the Metabolomic Society · 2020Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the urinary metabolomics for finding biomarkers in urine, owing to high concentrations of urea, for chromatography-based metabolomic analysis, urea needed to be degraded by urease. This urease pretreatment has been the key step of sample preparation for standard urinary metabolomics until today even for mass spectrometry-based analysis. The urease pretreatment involving incubation of urine with urease contradicts the concept of metabolome sampling, which should immediately arrest metabolic reactions to prevent alterations of a metabolite profile. Nonetheless, the impact of urease pretreatment has not been clearly elucidated yet. We found that activities of urease and endogenous urinary enzymes and metabolite contaminants from the urease preparations introduce artefacts into metabolite profiles, thus leading to misinterpretation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.