ArticlePlant biotechnology journal2020
Root-specific expression of chickpea cytokinin oxidase/dehydrogenase 6 leads to enhanced root growth, drought tolerance and yield without compromising nodulation.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Chickpea Biofortification for Cytokinin DehydrogenaseFrontiers in genetics · 2022Pooled it
- Advancing climate adaptation in saffron through CRISPR-based modulation of stress tolerance and photoperiodic flowering control.GM crops & food · 2026Review
- Evolutionary Repurposing of Cytokinin Signaling in Plant Development and Symbiosis.Plants (Basel, Switzerland) · 2026Review
- Physiological and transcriptome analyses for revealing the mechanism of adventitious root formation in Camellia luteoflora.BMC plant biology · 2026Article
- Root-specific cytokinin catabolism promotes Nicotiana root growth and resilience to drought stress.Planta · 2026Article
- Analysis of expression characteristics of oil palm EgPRP1 promoter as potential tools for root-trait modification.Plant cell reports · 2026Article
- Biotechnological modification of the cytokinin regulatory system to improve drought and heat tolerance in the major crops.Plant cell reports · 2026Review
- Multi-omics reveals DEAD-box RNA helicase 20 as key protein enhancing wilt resistance in transgenic chickpea.Plant cell reports · 2025Article
- Genome-wide investigation of cytokinin oxidase/dehydrogenase (CKX) family genes in Brassica juncea with an emphasis on yield-influencing CKX.Scientific reports · 2025Article
- Pangenome analysis reveals structural variation associated with seed size and weight traits in peanut.Nature genetics · 2025Article
- GmbZIP4a/b Positively Regulate Nodule Number by Affecting Cytokinin Biosynthesis inInternational journal of molecular sciences · 2024Article
- A gain-of-function mutation in BnaIAA13 disrupts vascular tissue and lateral root development in Brassica napus.Journal of experimental botany · 2024Article
- The F-Box Protein TaFBA1 Positively Regulates Drought Resistance and Yield Traits in Wheat.Plants (Basel, Switzerland) · 2024Article
- Identification of CKX gene family in Morus indica cv K2 and functional characterization of MiCKX4 during abiotic stress.Stress biology · 2024Article
- CaLAP1 and CaLAP2 orchestrate anthocyanin biosynthesis in the seed coat of Cicer arietinum.Planta · 2024Article
- Modulating root system architecture: cross-talk between auxin and phytohormones.Frontiers in plant science · 2024Review
- Loss-function mutants of OsCKX gene family based on CRISPR-Cas systems revealed their diversified roles in rice.The plant genome · 2023Article
- A superior gene allele involved in abscisic acid signaling enhances drought tolerance and yield in chickpea.Plant physiology · 2023Article
- Modifications of Phytohormone Metabolism Aimed at Stimulation of Plant Growth, Improving Their Productivity and Tolerance to Abiotic and Biotic Stress Factors.Plants (Basel, Switzerland) · 2022Review
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinin group of phytohormones regulate root elongation and branching during post-embryonic development. Cytokinin-degrading enzymes cytokinin oxidases/dehydrogenases (CKXs) have been deployed to investigate biological activities of cytokinin and to engineer root growth. We expressed chickpea cytokinin oxidase 6 (CaCKX6) under the control of a chickpea root-specific promoter of CaWRKY31 in Arabidopsis thaliana and chickpea having determinate and indeterminate growth patterns, respectively, to study the effect of cytokinin depletion on root growth and drought tolerance. Root-specific expression of CaCKX6 led to a significant increase in lateral root number and root biomass in Arabidopsis and chickpea without any penalty to vegetative and reproductive growth of shoot. Transgenic chickpea lines showed increased CKX activity in root. Soil-grown advanced chickpea transgenic lines exhibited higher root-to-shoot biomass ratio and enhanced long-term drought tolerance. These chickpea lines were not compromised in root nodulation and nitrogen fixation. The seed yield in some lines was up to 25% higher with no penalty in protein content. Transgenic chickpea seeds possessed higher levels of zinc, iron, potassium and copper. Our results demonstrated the potential of cytokinin level manipulation in increasing lateral root number and root biomass for agronomic trait improvement in an edible legume crop with indeterminate growth habit.
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