Evidence map›Paper›PMID 32180681›Full record

ArticleMolecular vision2020

A two-step method for identifying photopigment opsin and

Shari R Atilano, M Cristina Kenney, Adriana D Briscoe, Kimberly A Jameson

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Molecular vision, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Next-Generation Sequencing Applications for Inherited Retinal Diseases.International journal of molecular sciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Shari R AtilanoGavin Herbert Eye Institute, School of Medicine, University of California, Irvine, CA.
M Cristina KenneyGavin Herbert Eye Institute, School of Medicine, University of California, Irvine, CA.
Adriana D BriscoeDepartment of Ecology and Evolutionary Biology, University of California Irvine, Irvine, CA.
Kimberly A JamesonInstitute for Mathematical Behavioral Sciences, University of California Irvine, Irvine, CA.
University of California, Irvine · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Purpose: To present a detailed, reliable long range-PCR and sequencing (LR-PCR-Seq) procedure to identify human opsin gene sequences for variations in the long wavelength-sensitive ( Methods: Color vision was assessed for nine subjects using the Farnsworth-Munsell 100 hue test, Ishihara pseudoisochromatic plates, and the Rabin cone-contrast threshold procedure (ColorDX, Konan Medical). The color vision phenotypes were normal trichromacy (n = 3), potential tetrachromacy (n = 3), dichromacy (n = 2), and unexplained low color vision (n = 1). DNA was isolated from blood or saliva and LR-PCR amplified into individual products: Results: The LR-PCR-Seq technique identified known single nucleotide polymorphisms (SNPs) in Conclusions: This LR-PCR-Seq procedure (single PCR reaction per gene followed by sequencing) can identify exonic and intronic SNP variants in

Indexed as

AdultAged, 80 and overColor VisionExonsFemaleGenotypeHumansMaleMiddle AgedMutationOpsinsPhenotypePolymerase Chain ReactionPolymorphism, Single NucleotideRhodopsinRod Opsinslong-wavelength opsinmiddle-wavelength opsinOpsinsRhodopsinRod Opsinsshort-wavelength opsin

Identifiers

PMID32180681
PMCPMC7058431
OpenAlexW3011563942

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.