ArticleJournal of food science and technology2020
Ultrasonication-assisted extraction of a phytomelatonin-rich, erucic acid-lean nutraceutical supplement from mustard seeds: an antioxidant synergy in the extract by reductionism.
Article in Journal of food science and technology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 9 citations in OpenAlex.
- Comparative Analysis of Polyphenolic Acids from VariousFoods (Basel, Switzerland) · 2025Article
- Common Methods of Extraction and Determination of Phytomelatonin in Plants.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Melatonin from Microorganisms, Algae, and Plants as Possible Alternatives to Synthetic Melatonin.Metabolites · 2023Review
- Melatonin inMolecules (Basel, Switzerland) · 2022Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phytomelatonin-rich (194.02 ± 2.45-205.80 ± 1.67 ng/g of dry mustard seeds) and erucic acid-lean (below 2%) extracts from an oilseed crop, (yellow and black mustard seeds) have been successfully obtained by ultrasonication-assisted-extraction in ethanol-water. Fourier-transform infrared spectroscopy and liquid chromatography-mass spectrum analyses have confirmed the presence of phytomelatonin along with tocopherol, ascorbic acid, limonene and linalool in the extract. Field emission scanning electron micrographs confirmed the cavitational effects of sonication on mustard seed matrices. Electron paramagnetic resonance spectroscopy established the strong antioxidant activities (72.25-75.49%) of the extracts foregoing erroneous spectrophotometric result of pan assay interference compounds. A synergistic effect value of 1.13 (greater than unity) confirmed synergistic co-existence of the antioxidants in the extract. This study interestingly revealed that an antioxidant synergy could be obtained by classical reductionism. Acute oral toxicity of the extracts were found to be greater than 5000 mg/kg body weight of rats. The extracts are perfectly safe to be utilized as antioxidative food supplements.
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