ArticleeLife2020
Chemoptogenetic ablation of neuronal mitochondria in vivo with spatiotemporal precision and controllable severity.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Functional interrogation of neuronal connections by chemoptogenetic presynaptic ablation.Science advances · 2026Article
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
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- A human Tau expressing zebrafish model of progressive supranuclear palsy identifies Brd4 as a regulator of microglial synaptic elimination.Nature communications · 2024Article
- Full-field exposure of larval zebrafish to narrow waveband LED light sources at defined power and energy for optogenetic applications.Journal of neuroscience methods · 2024Article
- Article
- A Critical Review of Zebrafish Neurological Disease Models-1. The Premise: Neuroanatomical, Cellular and Genetic Homology and Experimental Tractability.Oxford open neuroscience · 2023Article
- Quantification of functional recovery in a larval zebrafish model of spinal cord injury.Journal of neuroscience research · 2022Article
- Targeting Mitochondrial Function with Chemoptogenetics.Biomedicines · 2022Review
- Targeted Formation of 8-Oxoguanine in Telomeres.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Sinusoidal analysis reveals a non-linear and dopamine-dependent relationship between ambient illumination and motor activity in larval zebrafish.Neuroscience letters · 2021Article
- An energetics perspective on geroscience: mitochondrial protonmotive force and aging.GeroScience · 2021Article
- Mechanism of Pacemaker Activity in Zebrafish DC2/4 Dopaminergic Neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- α-Synuclein amplifies cytoplasmic peroxide flux and oxidative stress provoked by mitochondrial inhibitors in CNS dopaminergic neurons in vivo.Redox biology · 2020Article
- Subcellular Singlet Oxygen and Cell Death: Location Matters.Frontiers in chemistry · 2020Article
Corrections and comments
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Authors and funding
15 authors at 4 institutions in 2 countries.
Funding
Abstract
Mitochondrial dysfunction is implicated in the pathogenesis of multiple neurological diseases, but elucidation of underlying mechanisms is limited experimentally by the inability to damage specific mitochondria in defined neuronal groups. We developed a precision chemoptogenetic approach to target neuronal mitochondria in the intact nervous system in vivo. MG2I, a chemical fluorogen, produces singlet oxygen when bound to the fluorogen-activating protein dL5** and exposed to far-red light. Transgenic zebrafish expressing dL5** within neuronal mitochondria showed dramatic MG2I- and light-dependent neurobehavioral deficits, caused by neuronal bioenergetic crisis and acute neuronal depolarization. These abnormalities resulted from loss of neuronal respiration, associated with mitochondrial fragmentation, swelling and elimination of cristae. Remaining cellular ultrastructure was preserved initially, but cellular pathology downstream of mitochondrial damage eventually culminated in neuronal death. Our work provides powerful new chemoptogenetic tools for investigating mitochondrial homeostasis and pathophysiology and shows a direct relationship between mitochondrial function, neuronal biogenetics and whole-animal behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.