Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model.

Emrah Kara, Nis Valentin Nielsen, Bergrun Eggertsdottir, Bernd Thiede, Sandip M Kanse, Geir Åge Løset

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Article in Chembiochem : a European journal of chemical biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.4field-weighted citation impact, top 34% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

Design and Characterization of a New pVII Combinatorial Phage Display Peptide Library for Protease Substrate Mining Using Factor VII Activating Protease (FSAP) as Model.Chembiochem : a European journal of chemical biology · 2020
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Emrah KaraInstitute of Basal Medical Sciences Oslo University Hospital, University of Oslo, Oslo, Norway.ORCID 0000-0001-9704-9190

Nis Valentin NielsenInstitute of Basal Medical Sciences Oslo University Hospital, University of Oslo, Oslo, Norway.ORCID 0000-0001-9260-6154

Bergrun EggertsdottirDepartment of Biosciences, University of Oslo, 0316, Oslo, Norway.

Bernd ThiedeDepartment of Biosciences, University of Oslo, 0316, Oslo, Norway.ORCID 0000-0002-2804-9522

Sandip M KanseInstitute of Basal Medical Sciences Oslo University Hospital, University of Oslo, Oslo, Norway.ORCID 0000-0003-0782-9957

Geir Åge LøsetDepartment of Biosciences, University of Oslo, 0316, Oslo, Norway.ORCID 0000-0002-5257-8571

Oslo University Hospital · NOUniversity of Oslo · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated.

Indexed as

Amino Acid SequenceChromatography, High Pressure LiquidHumansMass SpectrometryPeptide LibraryPeptidesSerine EndopeptidasesSubstrate SpecificityHABP2 protein, humanPeptide LibraryPeptidesSerine EndopeptidasesFSAPMarburg IPeptide affinity tagPhage displayProtease substrate specificity Protein−protein interactionspVII

Identifiers

PMID32180321

DOI10.1002/cbic.201900705

PMCPMC7383712

OpenAlexW3011512285

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.