ArticleScientific reports2020
Three-gene PCR and high-resolution melting analysis for differentiating vertebrate species mitochondrial DNA for biodiversity research and complementing forensic surveillance.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Mosquito-based xenosurveillance reveals circulation of Anaplasma and Neoehrlichia in livestock at a human-wildlife interface in Kenya.Parasites & vectors · 2026Article
- Species Identification of Sea Turtles in Thailand Using DNA Barcoding Coupled With High-Resolution Melting (Bar-HRM) Analysis.Scientifica · 2026Article
- Integrating portable qPCR and image recognition to combat illegal trade in sharks and rays.Scientific reports · 2025Article
- A multifaceted molecular approach to surveillance of leishmaniasis: Identification of sand fly species, Leishmania parasites, and blood meal sources using high-resolution melting analysis.PLoS neglected tropical diseases · 2025Article
- When DNA Tells the Tale: High-Resolution Melting as a Forensic Tool for Mediterranean Cetacean Identification.International journal of molecular sciences · 2025Article
- Limited knowledge of health risks along the illegal wild meat value chain in the Nairobi Metropolitan Area (NMA).PloS one · 2025Article
- Assessing the feasibility of free DNA for disaster victim identification and forensic applications.Scientific reports · 2024Article
- Putative bloodmeal sources in Glossina austeni tsetse fly of Arabuko Sokoke National Reserve in Kenya.PloS one · 2024Article
- Efficiency of mitochondrial genes and nuclear Alu elements in detecting human DNA in blood meals of Anopheles stephensi mosquitoes: a time-course study.Parasites & vectors · 2023Article
- A new multiplex qPCR assay to detect and differentiate big cat species in the illegal wildlife trade.Scientific reports · 2023Article
- Bloodmeal host identities among sympatricFrontiers in tropical diseases · 2023Article
- Tracking SARS-CoV-2 seropositivity in rural communities using blood-fed mosquitoes: a proof-of-concept study.Frontiers in epidemiology · 2023Article
- Turtles in Malaysia: A Review of Conservation Status and a Call for Research.Animals : an open access journal from MDPI · 2022Review
- Evidence of co-exposure with Brucella spp, Coxiella burnetii, and Rift Valley fever virus among various species of wildlife in Kenya.PLoS neglected tropical diseases · 2022Article
- Development and validation of simultaneous identification of 26 mammalian and poultry species by a multiplex assay.International journal of legal medicine · 2022Article
- Article
- Detection of Species Substitution in the Meat Value Chain by High-Resolution Melting Analysis of Mitochondrial PCR Products.Foods (Basel, Switzerland) · 2021Article
- Tsetse Bloodmeal Analyses Incriminate the Common WarthogPathogens (Basel, Switzerland) · 2021Article
- Review
- Feasibility of melting fingerprint obtained from ISSR-HRM curves for marine mammal species identification.PeerJ · 2021Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Reliable molecular identification of vertebrate species from morphologically unidentifiable tissue is critical for the prosecution of illegally-traded wildlife products, conservation-based biodiversity research, and identification of blood-meal hosts of hematophagous invertebrates. However, forensic identification of vertebrate tissue relies on sequencing of the mitochondrial cytochrome oxidase I (COI) 'barcode' gene, which remains costly for purposes of screening large numbers of unknown samples during routine surveillance. Here, we adapted a rapid, low-cost approach to differentiate 10 domestic and 24 wildlife species that are common in the East African illegal wildlife products trade based on their unique high-resolution melting profiles from COI, cytochrome b, and 16S ribosomal RNA gene PCR products. Using the approach, we identified (i) giraffe among covertly sampled meat from Kenyan butcheries, and (ii) forest elephant mitochondrial sequences among savannah elephant reference samples. This approach is being adopted for high-throughput pre-screening of potential bushmeat samples in East African forensic science pipelines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.