ArticleScientific reports2020
Proximity-dependent biotin labelling reveals CP190 as an EcR/Usp molecular partner.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- SRP/dGATAb modulates the ecdysone response bidirectionally via locus-specific regulatory landscape.Epigenetics & chromatin · 2025Article
- Adaptation of the Protocol for the Isolation of Biotinylated Protein Complexes forInternational journal of molecular sciences · 2025Article
- Vostok: A looping factor for the organization of the regulatory genome in the Drosophila brain.Molecular cell · 2025Article
- Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation.Nucleic acids research · 2025Article
- Development and Validation of a Proximity Labeling Fusion Protein Construct to Identify the Protein-Protein Interactions of Transcription Factors.Methods in molecular biology (Clifton, N.J.) · 2025Article
- SetDB1 and Su(var)3-9 are essential for late stages of larval development of Drosophila melanogaster.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Article
- The MADF-BESS Protein CP60 Is Recruited to Insulators via CP190 and Has Redundant Functions inInternational journal of molecular sciences · 2023Article
- Coregulators Reside withinInternational journal of molecular sciences · 2023Article
- Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins.Cellular and molecular life sciences : CMLS · 2022Article
- Mechanisms of CP190 Interaction with Architectural Proteins in Drosophila Melanogaster.International journal of molecular sciences · 2021Article
- Antibodies as Biosensors' Key Components: State-of-the-Art in Russia 2020-2021.Sensors (Basel, Switzerland) · 2021Review
- Anatomy and evolution of a DNA replication origin.Chromosoma · 2021Article
- Mechanism and functional role of the interaction between CP190 and the architectural protein Pita in Drosophila melanogaster.Epigenetics & chromatin · 2021Article
- The negative elongation factor NELF promotes induced transcriptional response of Drosophila ecdysone-dependent genes.Scientific reports · 2021Article
- Constructing and Tuning Excitatory Cholinergic Synapses: The Multifaceted Functions of Nicotinic Acetylcholine Receptors inFrontiers in cellular neuroscience · 2021Review
- Proximity Labeling Techniques to Study Chromatin.Frontiers in genetics · 2020Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Proximity-dependent biotin labelling revealed undescribed participants of the ecdysone response in Drosophila. Two labelling enzymes (BioID2 and APEX2) were fused to EcR or Usp to biotin label the surrounding proteins. The EcR/Usp heterodimer was found to collaborate with nuclear pore subunits, chromatin remodelers, and architectural proteins. Many proteins identified through proximity-dependent labelling with EcR/Usp were described previously as functional components of an ecdysone response, corroborating the potency of this labelling method. A link to ecdysone response was confirmed for some newly discovered regulators by immunoprecipitation of prepupal nuclear extract with anti-EcR antibodies and functional experiments in Drosophila S2 cells. A more in-depth study was conducted to clarify the association of EcR/Usp with one of the detected proteins, CP190, a well-described cofactor of Drosophila insulators. CP190 was found to co-immunoprecipitate with the EcR subunit of EcR/Usp in a 20E-independent manner. ChIP-Seq experiments revealed only partial overlapping between CP190 and EcR bound sites in the Drosophila genome and complete absence of CP190 binding at 20E-dependent enhancers. Analysis of Hi-C data demonstrated an existence of remote interactions between 20E-dependent enhancers and CP190 sites which suggests formation of a protein complex between EcR/Usp and CP190 through the space. Our results support the previous concept that CP190 has a role in stabilization of specific chromatin loops for proper activation of transcription of genes regulated by 20E hormone.
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