ArticleJournal of neuroscience methods2020
MicroCT optimisation for imaging fascicular anatomy in peripheral nerves.
Article in Journal of neuroscience methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 51 citations in OpenAlex.
- Electroacupuncture Enhances Effectiveness of Omeprazole in Indomethacin-Induced Gastric Injury Mice via Nrf2 Activation and Vagus Nerve Conduction.Chinese journal of integrative medicine · 2026Article
- Automated 3D segmentation of human vagus nerve fascicles and epineurium from micro-computed tomography images using anatomy-aware neural networks.Journal of neural engineering · 2026Article
- Emergent technologies in clinical neurophysiology to study the neuromuscular system: IFCN handbook chapter.Clinical neurophysiology practice · 2026Review
- A Novel Atraumatic Polymer-Assisted Peripheral Nerve Repair Device Compared With Microsurgical Neurorrhaphy.Journal of hand surgery global online · 2025Article
- More Than Epineurium Deep: Characterizing Peripheral Nerve Damage Using High-Resolution Micro-Computed Tomography for Simulated Peripheral Nerve Lacerations.Journal of hand surgery global online · 2025Article
- Revealing the Three-Dimensional Complexity of Facial Anatomy Through Micro-Computed Tomography.Journal of cosmetic dermatology · 2025Article
- Scaling of vagus nerve stimulation parameters does not achieve equivalent nerve responses across species.Bioelectronic medicine · 2025Article
- Hydrogel Adhesive Integrated-Microstructured Electrodes for Cuff-Free, Less-Invasive, and Stable Interface for Vagus Nerve Stimulation.Advanced healthcare materials · 2025Article
- Anatomical Variations in the Formation of the Sural Nerve: A Pilot Study in a Sample of Lithuanian Cadavers.Medicina (Kaunas, Lithuania) · 2025Article
- Fascicle differentiation of upper extremity nerves on high-resolution ultrasound with multimodal microscopic verification.Scientific reports · 2025Article
- Computational modeling of autonomic nerve stimulation: Vagus et al.Current opinion in biomedical engineering · 2024Article
- Morphological Variability of the Sural Nerve and Its Clinical Significance.Journal of clinical medicine · 2024Review
- Vagal nerve stimulation in myocardial ischemia/reperfusion injury: from bench to bedside.Bioelectronic medicine · 2024Review
- Multi-Channel Microscale Nerve Cuffs for Spatially Selective Neuromodulation.Micromachines · 2024Article
- NerveTracker: a Python-based software toolkit for visualizing and tracking groups of nerve fibers in serial block-face microscopy with ultraviolet surface excitation images.Journal of biomedical optics · 2024Article
- Advanced neuroprosthetic electrode design optimized by electromagnetic finite element simulation: innovations and applications.Frontiers in bioengineering and biotechnology · 2024Review
- Selective peripheral nerve recording using simulated human median nerve activity and convolutional neural networks.Biomedical engineering online · 2023Article
- Deep-learning segmentation of fascicles from microCT of the human vagus nerve.Frontiers in neuroscience · 2023Article
- Organotopic organization of the porcine mid-cervical vagus nerve.Frontiers in neuroscience · 2023Article
- Fascicles split or merge every ∼560 microns within the human cervical vagus nerve.Journal of neural engineering · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundDue to the lack of understanding of the fascicular organisation, vagus nerve stimulation (VNS) leads to unwanted off-target effects. Micro-computed tomography (microCT) can be used to trace fascicles from periphery and image fascicular anatomy. NEW
methodIn this study, we present a simple and reproducible method for imaging fascicles in peripheral nerves with iodine staining and microCT for the determination of fascicular anatomy and organisation.
resultsAt the determined optimal pre-processing steps and scanning parameters, the microCT protocol allowed for segmentation and tracking of fascicles within the nerves. This was achieved after 24 hours and 120 hours of staining with Lugol's solution (1% total iodine) for rat sciatic and pig vagus nerves, respectively, and the following scanning parameters: 4 μm voxel size, 35 kVp energy, 114 μA current, 4 W power, 0.25 fps in 4 s exposure time, 3176 projections and a molybdenum target. COMPARISON WITH EXISTING METHOD(S): This optimised method for imaging fascicles provides high-resolution, three-dimensional images and full imaging penetration depth not obtainable with methods typically used such as histology, magnetic resonance imaging and optical coherence tomography whilst obviating time-consuming pre-processing methods, the amount of memory required, destruction of the samples and the cost associated with current microCT methods.
conclusionThe optimised microCT protocol facilitates segmentation and tracking of the fascicles within the nerve. The resulting segmentation map of the functional anatomical organisation of the vagus nerve will enable selective VNS ultimately allowing for the avoidance of the off-target effects and improving its therapeutic efficacy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.